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Literature summary extracted from

  • Dumon, C.; Varvak, A.; Wall, M.A.; Flint, J.E.; Lewis, R.J.; Lakey, J.H.; Morland, C.; Luginbuehl, P.; Healey, S.; Todaro, T.; DeSantis, G.; Sun, M.; Parra-Gessert, L.; Tan, X.; Weiner, D.P.; Gilbert, H.J.
    Engineering hyperthermostability into a GH11 xylanase is mediated by subtle changes to protein structure (2008), J. Biol. Chem., 283, 22557-22564.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 amplified fragment of Xyn11 (nucleotides 64-646) cloned into the pSE420-CHis vector and transformed into Escherichia coli strain XL1-Blue MR uncultured bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.8 wild-type Xyn11 and hyperthermostable mutant Xyn11TS crystallized to a resolution of 1.8 and 1.9 A, respectively. Crystal structure of Xyn11TS is extremely similar to the wild-type. Crystals of Xyn11TS belong to space group C2, with unit cell dimensions of a=64.7 A, b=33.6 A, c=83.1 A, alpha=90.0°, beta=101.9°, gamma=90.0°, and with one molecule occupying the crystallographic asymmetric unit. Crystals of Xyn11 belong to space group P212121, with unit cell dimensions of a=36.3 A, b=63.2 A, c=75.1 A, alpha=beta=gamma=90.0°, also with one molecule occupying the crystallographic asymmetric unit uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 N14H thermostabilizing mutation, catalytic activity is broadly similar to the wild-type uncultured bacterium
3.2.1.8 N30V thermostabilizing mutation uncultured bacterium
3.2.1.8 Q10S thermostabilizing mutation uncultured bacterium
3.2.1.8 Q34C thermostabilizing mutation uncultured bacterium
3.2.1.8 Q34H thermostabilizing mutation uncultured bacterium
3.2.1.8 Q34L thermostabilizing mutation, catalytic activity is broadly similar to the wild-type uncultured bacterium
3.2.1.8 S194H thermostabilizing mutation uncultured bacterium
3.2.1.8 S25E thermostabilizing mutation uncultured bacterium
3.2.1.8 S35E thermostabilizing mutation, catalytic activity is broadly similar to the wild-type uncultured bacterium
3.2.1.8 S71T thermostabilizing mutation, catalytic activity is broadly similar to the wild-type uncultured bacterium
3.2.1.8 S9P locks the conformation of a surface loop, catalytic activity is broadly similar to the wild-type uncultured bacterium
3.2.1.8 S9P thermostabilizing mutation uncultured bacterium
3.2.1.8 S9P/T13F/N14H/Y18F/Q34L/S35E/S71T hyperthermostable mutant Xyn11TS, retains full activity after incubation at 90°C for 60 min, catalytic activity is broadly similar to the wild-type uncultured bacterium
3.2.1.8 T13F increases hydrophobic interactions uncultured bacterium
3.2.1.8 T13F thermostabilizing mutation, catalytic activity is broadly similar to the wild-type uncultured bacterium
3.2.1.8 T13Y thermostabilizing mutation uncultured bacterium
3.2.1.8 T4L thermostabilizing mutation uncultured bacterium
3.2.1.8 Y18F thermostabilizing mutation, catalytic activity is broadly similar to the wild-type uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 uncultured bacterium B2LWN3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 mutants and full-length protein Xyn11 purified by cation exchange chromatography and gel filtration, to more than 95% electrophoretic purity uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-nitrophenyl beta-D-xylotrioside + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.8 oat-spelt xylan + H2O
-
 uncultured bacterium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 GH11 xylanase
-
 uncultured bacterium
3.2.1.8 xylanase
-
 uncultured bacterium
3.2.1.8 Xyn11
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 65
-
 native enzyme uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
 Xyn11 in its apo form, is not subject to thermal inactivation up to 60°C uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 2.317
-
 oat-spelt xylan hyperthermostable mutant Xyn11TS uncultured bacterium
3.2.1.8 2.583
-
 oat-spelt xylan wild-type uncultured bacterium
3.2.1.8 2.583
-
 oat-spelt xylan mutant S71T uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7.2 8 recombinant form of full-length Xyn11, pH optimum of 8.0 using oat-spelled xylan and 7.2 when 4-nitrophenyl-beta-D-xylotrioside is the substrate uncultured bacterium