Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gladyshev, V.N.; Boyington, J.C.; Khangulov, S.V.; Grahame, D.A.; Stadtman, T.C.; Sun, P.D.
    Characterization of crystalline formate dehydrogenase H from Escherichia coli (1996), J. Biol. Chem., 271, 8095-8100.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.98.4 crystals diffract to 2.6 A resolution and belong to a space group of P4(1)2(1)2 or P4(3)2(1)2 with unit cell dimensions a = b = 146.1 A and c = 82.7 A. There is one monomer of FDH per crystallographic asymmetric unit. Similar diffraction quality crystals of oxidized FDH can be obtained by oxidation of crystals of formate-reduced enzyme with benzyl viologen. Mo(IV)- and the reduced FeS cluster containing form of the enzyme was crystallized and this can be converted into Mo(VI)- and oxidized FeS cluster form upon oxidation Escherichia coli

General Stability

EC Number General Stability Organism
1.17.98.4 enzyme in dilute solutions (30 mg/ml) is rapidly inactivated at basic pH or in the presence of formate under anaerobic conditions, but at higher enzyme concentrations (3 mg/ml) the enzyme is relatively stable Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.98.4 Fe Mo(IV)- and the reduced FeS cluster-containing form of the enzyme is crystallized and this can be converted into Mo(VI)- and oxidized FeS cluster form upon oxidation Escherichia coli
1.17.98.4 Mo Mo(IV)- and the reduced FeS cluster-containing form of the enzyme is crystallized and this can be converted into Mo(VI)- and oxidized FeS cluster form upon oxidation Escherichia coli
1.17.98.4 Se selenocysteine-containing enzyme. The molybdenum-coordinated selenocysteine is essential for catalytic activity of the native enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.98.4 80000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.17.98.4 Escherichia coli
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.98.4 the formate-reduced enzyme is extremely sensitive to air inactivation Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.98.4
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.98.4 formate + benzyl viologen
-
 Escherichia coli CO2 + reduced benzyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.98.4 ? x * 80000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.17.98.4 formate dehydrogenase H
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.98.4 8
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.98.4 6
-
 maximum stability Escherichia coli