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Literature summary extracted from

  • Kuhm, A.E.; Knackmuss, H.J.; Stolz, A.
    Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates (1993), J. Biol. Chem., 268, 9484-9489.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.2.45 -16°C, 4°C or 20°C, the enzyme is very stable in cell free extracts, after a 1-week incubation (protein concentration: 2.6 mg/ml) in Na/K-phosphate buffer (pH 7.3, 50 mM) the initial enzyme activity is completely recovered Sphingobium xenophagum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.45 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 Sphingobium xenophagum
4.1.2.45 additional information no inhibition: benzylideneacetone, cinnamic acid Sphingobium xenophagum
4.1.2.45 p-chloromercuribenzoate enzyme is partially reactivated by addition of dithiothreitol Sphingobium xenophagum
4.1.2.45 Salicylaldehyde
-
 Sphingobium xenophagum
4.1.2.45 sodium borhydride
-
 Sphingobium xenophagum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.45 0.006
-
 (3E)-4-(2,6-dihydroxyphenyl)-2-oxobut-3-enoate pH 7.0 Sphingobium xenophagum
4.1.2.45 0.015
-
 (3E)-4-(2,4-dihydroxyphenyl)-2-oxobut-3-enoate pH 7.0 Sphingobium xenophagum
4.1.2.45 0.017
-
 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate pH 7.0 Sphingobium xenophagum
4.1.2.45 0.4
-
 benzylidenepyruvate pH 7.0 Sphingobium xenophagum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.45 38500
-
 3 * 38500, SDS-PAGE Sphingobium xenophagum
4.1.2.45 120000
-
 gel filtration Sphingobium xenophagum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.45 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O Sphingobium xenophagum this reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria salicylaldehyde + pyruvate
-
 ?
4.1.2.45 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O Sphingobium xenophagum BN6 this reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria salicylaldehyde + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.45 Sphingobium xenophagum
-
 BN6
-
4.1.2.45 Sphingobium xenophagum BN6
-
 BN6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.45
-
 Sphingobium xenophagum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.45 23.67
-
-
 Sphingobium xenophagum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.45 (3E)-4-(2,4-dihydroxyphenyl)-2-oxobut-3-enoate + H2O
-
 Sphingobium xenophagum 2,4-dihydroxybenzaldehyde + pyruvate
-
 ?
4.1.2.45 (3E)-4-(2,4-dihydroxyphenyl)-2-oxobut-3-enoate + H2O
-
 Sphingobium xenophagum BN6 2,4-dihydroxybenzaldehyde + pyruvate
-
 ?
4.1.2.45 (3E)-4-(2,6-dihydroxyphenyl)-2-oxobut-3-enoate + H2O
-
 Sphingobium xenophagum 2,6-dihydroxybenzaldehyde + pyruvate
-
 ?
4.1.2.45 (3E)-4-(2,6-dihydroxyphenyl)-2-oxobut-3-enoate + H2O
-
 Sphingobium xenophagum BN6 2,6-dihydroxybenzaldehyde + pyruvate
-
 ?
4.1.2.45 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O this reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria Sphingobium xenophagum salicylaldehyde + pyruvate
-
 ?
4.1.2.45 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-hydroxybenzylidinepyruvate, i.e. 2'-hydroxybenzalpyruvate, i.e. trans-O-hydroxybenzylidenepyruvate, intermediate formation of a stable Schiff base between enzyme and substrate Sphingobium xenophagum salicylaldehyde + pyruvate salicylaldehyde i.e. 2-hydroxybenzaldehyde ?
4.1.2.45 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O this reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria Sphingobium xenophagum BN6 salicylaldehyde + pyruvate
-
 ?
4.1.2.45 (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-hydroxybenzylidinepyruvate, i.e. 2'-hydroxybenzalpyruvate, i.e. trans-O-hydroxybenzylidenepyruvate, intermediate formation of a stable Schiff base between enzyme and substrate Sphingobium xenophagum BN6 salicylaldehyde + pyruvate salicylaldehyde i.e. 2-hydroxybenzaldehyde ?
4.1.2.45 benzylidenepyruvate + H2O with 1% of the activity found with (E)-2'-hydroxybenzylidinepyruvate Sphingobium xenophagum benzaldehyde + pyruvate
-
 ?
4.1.2.45 additional information no activity with: benzylideneacetone and cinnamic acid Sphingobium xenophagum ?
-
 ?
4.1.2.45 additional information no activity with: benzylideneacetone and cinnamic acid Sphingobium xenophagum BN6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.45 trimer 3 * 38500, SDS-PAGE Sphingobium xenophagum

Synonyms

EC Number Synonyms Comment Organism
4.1.2.45 2'-hydroxybenzalpyruvate aldolase
-
 Sphingobium xenophagum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.45 8
-
 in Tris/HCl or Na/K-phosphate buffer Sphingobium xenophagum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.45 6 9 more than 80% of the maximal activity at pH 6.0 and pH 9.0, pH 5.0: 18% of maximal activity, pH 10.0: 14% of maximal activity Sphingobium xenophagum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.45 0.013
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.0 Sphingobium xenophagum