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Literature summary extracted from

  • Stroeman, P.; Reinert, W.R.; Giles, N.H.
    Purification and characterization of 3-dehydroshikimate dehydratase, an enzyme in the inducible quinic acid catabolic pathway of Neurospora crassa (1978), J. Biol. Chem., 253, 4593-4598.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.118 EDTA presence of Mg2+ reactivates Neurospora crassa
4.2.1.118 K+ weak Neurospora crassa
4.2.1.118 Na+ weak Neurospora crassa
4.2.1.118 NH4+ weak Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.118 0.00059
-
 3-dehydroshikimate pH 7.5, 37°C Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.118 Ba2+ activation Neurospora crassa
4.2.1.118 Co2+ activation Neurospora crassa
4.2.1.118 Mg2+ up to 35fold activation. Presence at 25 mM protects from thermal inactivation Neurospora crassa
4.2.1.118 Mn2+ activation Neurospora crassa
4.2.1.118 Mo2+ activation Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.118 36500
-
 gel filtration Neurospora crassa
4.2.1.118 37000
-
 SDS-PAGE Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.118 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.118
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.118 257
-
 pH 7.5, 37°C Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
4.2.1.118 -20°C, loss of 50% of activity within 3 days Neurospora crassa
4.2.1.118 0-2°C, presence of 25 mM Mg2+, one week, less than 50% loss of activity Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.118 3-dehydroshikimate
-
 Neurospora crassa protocatechuate + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.118 47
-
 half-life below 12 min, presence of 25 mM Mg2+ extends the half-life to 220 min Neurospora crassa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.118 Neurospora crassa minor component, and 5.0, minor component
-
 4.8
4.2.1.118 Neurospora crassa major component, and 4.8, minor component
-
 5