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Literature summary extracted from

  • Bashir, Q.; Rashid, N.; Jamil, F.; Imanaka, T.; Akhtar, M.
    Highly thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Thermococcus kodakaraensis (2009), J. Biochem., 146, 95-102.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.103 plasmid pTZ-tdh transformed in Escherichia coli DH5alpha competent cells. NcoI-EcoRI restriction fragment inserted into the pET-8c expression vector at the corresponding sites. The resulting plasmid pET-tdh expressed in Escherichia coli strain BL21(DE3)CodonPlus-RIL Thermococcus kodakarensis KOD1

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.103 EDTA retains 65% of its original activity after dialysis at 48°C against a buffer containing 1 mM EDTA. Activity is lost when TDH is heated at 60°C for 40 min and in boiling water for 5 min in the presence of 1 mM EDTA Thermococcus kodakarensis KOD1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 0.028
-
 NAD+ at 50°C Thermococcus kodakarensis KOD1
1.1.1.103 1.6
-
 L-threonine at 50°C Thermococcus kodakarensis KOD1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.103 Ca2+ 0.1 mM reactivates EDTA-100% inhibited enzyme by 40% Thermococcus kodakarensis KOD1
1.1.1.103 Co2+ 0.1 mM reactivates EDTA-100% inhibited enzyme by 54% Thermococcus kodakarensis KOD1
1.1.1.103 Cu2+ 0.1 mM reactivates EDTA-100% inhibited enzyme by 63% Thermococcus kodakarensis KOD1
1.1.1.103 Mg2+ 0.1 mM reactivates EDTA-100% inhibited enzyme by 27% Thermococcus kodakarensis KOD1
1.1.1.103 Mn2+ 0.1 mM reactivates EDTA-100% inhibited enzyme by 38% Thermococcus kodakarensis KOD1
1.1.1.103 Ni2+ 0.1 mM reactivates EDTA-100% inhibited enzyme by 30% Thermococcus kodakarensis KOD1
1.1.1.103 Zn2+ four zinc-binding cysteine residues, 0.1 mM reactivates EDTA-100% inhibited enzyme by 75%, 0.05 mM reactivates by 100% Thermococcus kodakarensis KOD1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 38100
-
 sequence analysis Thermococcus kodakarensis KOD1
1.1.1.103 154000
-
 by gel filtration Thermococcus kodakarensis KOD1

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Thermococcus kodakarensis KOD1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 by heat treatment (about 80% pure recombinant TDH protein) and gel filtration Thermococcus kodakarensis KOD1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 L-serine + NAD+
-
 Thermococcus kodakarensis KOD1 L-2-amino-3-oxopropionate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+
-
 Thermococcus kodakarensis KOD1 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.103 L-threonine dehydrogenase
-
 Thermococcus kodakarensis KOD1
1.1.1.103 TDH
-
 Thermococcus kodakarensis KOD1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.103 90
-
-
 Thermococcus kodakarensis KOD1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.103 85
-
 the enzyme shows a half life of 2 h at 85°C and 24 min in boiling water Thermococcus kodakarensis KOD1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.103 12
-
-
 Thermococcus kodakarensis KOD1