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Literature summary extracted from

  • Matsuda, Y.; Inamori, K.; Osaki, T.; Eguchi, A.; Watanabe, A.; Kawabata, S.; Iba, K.; Arata, H.
    Nitric oxide-reductase homologue that contains a copper atom and has cytochrome c-oxidase activity from an aerobic phototrophic bacterium Roseobacter denitrificans (2002), J. Biochem., 131, 791-800.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.5 additional information oxidation of NADH by membranes is inhibited almost completely by antimycin A or myxothiazole, indicating that the bacterium does not have a quinol oxidase Roseobacter denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.5 Cu2+ protein contains heme c, heme b, and copper in a 1:2:1 stoichiometry Roseobacter denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.5 17600
-
 calculated, small subunit Roseobacter denitrificans
1.7.2.5 18000
-
 composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 Roseobacter denitrificans
1.7.2.5 37000
-
 composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 Roseobacter denitrificans
1.7.2.5 49000
-
 calculated, large subunit Roseobacter denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Roseobacter denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.5 DEAE-Sepharose CL-6B anion-exchange chromatography, Sephacryl S-300 gel filtration, and Mono Q anion-exchange chromatography. Enzyme is solubilized with sucrose monodecanoate from the membranes of Roseobacter denitrificans grown aerobically under light conditions, and purified to electrophoretic homogeneity Roseobacter denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.5 7.3
-
 last purification step Roseobacter denitrificans

Subunits

EC Number Subunits Comment Organism
1.7.2.5 dimer composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 Roseobacter denitrificans

Synonyms

EC Number Synonyms Comment Organism
1.7.2.5 nitric oxide-reductase NorBC homologue, cytochrome cb-type enzyme with cytochrome c-oxidase activity Roseobacter denitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.5 25
-
 assay at Roseobacter denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 heme protein contains heme c, heme b, and copper in a 1:2:1 stoichiometry Roseobacter denitrificans