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Literature summary extracted from
- CDP-alcohol hydrolase, a very efficient activity of the 5-nucleotidase/UDP-sugar hydrolase encoded by the ushA gene of Yersinia intermedia and Escherichia coli (2008), J. Bacteriol., 190, 6153-6161.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.1.45 | gene ushA, DNA and amino acid sequence determination and analysis, expression analysis by RT-PCR | Yersinia intermedia |
| 3.6.1.45 | gene ushA, overexpression of the enzyme | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.6.1.45 | additional information | ushA knockouts are unable to grow on AMP as the only carbon source and are significantly delayed or impaired in their growth on other purine nucleotides. Growth on nonadenine purine nucleotides is made possible by an acid phosphatase encoded by the aphA gene, which at neutral pH acts as an efficient 5'-nucleotidase of purine nucleotides, except AMP. Double knockouts in ushA and aphA do not grow on nucleotides AMP | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.45 | additional information | - |
additional information | kinetics of all the enzyme activites, overview | Escherichia coli | |
| 3.6.1.45 | additional information | - |
additional information | kinetics of all the enzyme activites, overview | Yersinia intermedia | |
| 3.6.1.45 | 0.0096 | - |
UDP-glucose | pH 7.5, 37°C | Yersinia intermedia |  |
| 3.6.1.45 | 0.0104 | - |
UDP-glucose | pH 7.5, 37°C | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.45 | Mg2+ | - |
Escherichia coli | |
| 3.6.1.45 | Mg2+ | - |
Yersinia intermedia | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.45 | 23000 | - |
x * 63000, band 1, SDS-PAGE, x * 23000, band 2, SDS-PAGE | Yersinia intermedia |
| 3.6.1.45 | 52000 | - |
gel filtration | Yersinia intermedia |
| 3.6.1.45 | 63000 | - |
x * 63000, band 1, SDS-PAGE, x * 23000, band 2, SDS-PAGE | Yersinia intermedia |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.45 | UDP-glucose + H2O | Yersinia intermedia | - |
UMP + alpha-D-glucose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-glucose + H2O | Escherichia coli | UshA protein, with 5'-nucleotidase and UDP-sugar hydrolase activities, is required for growth on AMP or UDP-glucose as sole sources of phosphorus | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-glucose + H2O | Yersinia intermedia CECT 7230 | - |
UMP + alpha-D-glucose 1-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.45 | Escherichia coli | - |
gene ushA | - |
| 3.6.1.45 | Yersinia intermedia | A4URQ8 | gene ushA | - |
| 3.6.1.45 | Yersinia intermedia CECT 7230 | A4URQ8 | gene ushA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.45 | UshA from suspension cell culture by gel filtration, dialysis, and anion exchange chromatography for enzyme characterization, and also by another adsorption chromatography step for peptide mass fingerprinting | Yersinia intermedia |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.6.1.45 | cell suspension culture | the CDP-hydrolase activity is released on Triton X-100-containing medium. Growth at 25°C is deemed optimum in terms of the enzyme-activity yield | Yersinia intermedia | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.45 | additional information | the enzyme shows UDP-sugar hydrolase EC 3.6.1.45, dinucleoside-polyphosphate hydrolase, CDP-alcohol hydrolase EC 3.6.1.53, and 5'-nucleosidase activities EC 3.1.3.5, it has a variety of activities and structure-function relationships, substrate specificity, overview | Escherichia coli | ? | - |
? | |
| 3.6.1.45 | additional information | the enzyme shows UDP-sugar hydrolase EC 3.6.1.45, dinucleoside-polyphosphate hydrolase, CDP-alcohol hydrolase EC 3.6.1.53, and 5'-nucleosidase activities EC 3.1.3.5, it has a variety of activities and structure-function relationships, substrate specificity, overview | Yersinia intermedia | ? | - |
? | |
| 3.6.1.45 | additional information | the enzyme shows UDP-sugar hydrolase EC 3.6.1.45, dinucleoside-polyphosphate hydrolase, CDP-alcohol hydrolase EC 3.6.1.53, and 5'-nucleosidase activities EC 3.1.3.5, it has a variety of activities and structure-function relationships, substrate specificity, overview | Yersinia intermedia CECT 7230 | ? | - |
? | |
| 3.6.1.45 | UDP-glucose + H2O | - |
Escherichia coli | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-glucose + H2O | - |
Yersinia intermedia | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-glucose + H2O | - |
Yersinia intermedia | UMP + alpha-D-glucose 1-phosphate | products are uridine, phosphate and D-glucose 1-phosphate. The phosphoanhydride and the nucleoside 5'-ester linkages are hydrolyzed without detectable signs of nucleoside monophosphate as an intermediate, possibly because it is hydrolyzed as soon as it is formed without leaving the active center | ? | |
| 3.6.1.45 | UDP-glucose + H2O | UshA protein, with 5'-nucleotidase and UDP-sugar hydrolase activities, is required for growth on AMP or UDP-glucose as sole sources of phosphorus | Escherichia coli | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-glucose + H2O | - |
Yersinia intermedia CECT 7230 | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| 3.6.1.45 | UDP-glucose + H2O | - |
Yersinia intermedia CECT 7230 | UMP + alpha-D-glucose 1-phosphate | products are uridine, phosphate and D-glucose 1-phosphate. The phosphoanhydride and the nucleoside 5'-ester linkages are hydrolyzed without detectable signs of nucleoside monophosphate as an intermediate, possibly because it is hydrolyzed as soon as it is formed without leaving the active center | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.45 | ? | x * 63000, band 1, SDS-PAGE, x * 23000, band 2, SDS-PAGE | Yersinia intermedia |
| 3.6.1.45 | More | UshA peptide mass fingerprint, overview | Yersinia intermedia |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.1.45 | 5'-nucleotidase/UDP-sugar hydrolase | - |
Escherichia coli |
| 3.6.1.45 | 5'-nucleotidase/UDP-sugar hydrolase | - |
Yersinia intermedia |
| 3.6.1.45 | UshA | - |
Escherichia coli |
| 3.6.1.45 | UshA | - |
Yersinia intermedia |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.45 | 37 | - |
assay at | Escherichia coli |
| 3.6.1.45 | 37 | - |
assay at | Yersinia intermedia |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.45 | 71 | - |
UDP-glucose | pH 7.5, 37°C | Escherichia coli | |
| 3.6.1.45 | 97 | - |
UDP-glucose | pH 7.5, 37°C | Yersinia intermedia | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.45 | 7.5 | - |
assay at | Escherichia coli |
| 3.6.1.45 | 7.5 | - |
assay at | Yersinia intermedia |
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