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Literature summary extracted from

  • Siddiqui, R.A.; Warnecke-Eberz, U.; Hengsberger, A.; Schneider, B.; Kostka, S.; Friedrich, B.
    Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16 (1993), J. Bacteriol., 175, 5867-5876.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.9.6.1 structural genes, napA and napB, are cloned, and their nucleotide sequences is determined Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
1.9.6.1 additional information the physiological role of NAP in nitrate metabolism is investigated in studies with a mutant bearing a transposon insertion in one of the structural genes Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9.6.1 0.12
-
 nitrate pH 5.5, 37°C Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.9.6.1 periplasm
-
 Cupriavidus necator
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.9.6.1 17000
-
 1 * 17000 + 1 * 87000, SDS-PAGE Cupriavidus necator
1.9.6.1 18924
-
 1 * 93309 + 1 * 18924, calculated from sequence Cupriavidus necator
1.9.6.1 87000
-
 1 * 17000 + 1 * 87000, SDS-PAGE Cupriavidus necator
1.9.6.1 92000
-
 gel filtration Cupriavidus necator
1.9.6.1 93309
-
 1 * 93309 + 1 * 18924, calculated from sequence Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.9.6.1 additional information Cupriavidus necator an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism ?
-
 ?
1.9.6.1 additional information Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.9.6.1 Cupriavidus necator
-
-
-
1.9.6.1 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.9.6.1
-
 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.9.6.1 additional information an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism Cupriavidus necator ?
-
 ?
1.9.6.1 additional information an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
1.9.6.1 nitrate + reduced benzyl viologen
-
 Cupriavidus necator nitrite + oxidized benzyl viologen + H2O
-
 ?
1.9.6.1 nitrate + reduced benzyl viologen
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 nitrite + oxidized benzyl viologen + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.9.6.1 heterodimer 1 * 17000 + 1 * 87000, SDS-PAGE Cupriavidus necator
1.9.6.1 heterodimer 1 * 93309 + 1 * 18924, calculated from sequence Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
1.9.6.1 NAP
-
 Cupriavidus necator
1.9.6.1 nitrate reductase, periplasmic
-
 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.9.6.1 37
-
 assay at Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.9.6.1 5.5
-
 assay at Cupriavidus necator