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Literature summary extracted from
- Molecular typing of industrial strains of Pseudomonas spp. isolated from milk and genetical and biochemical characterization of an extracellular protease produced by one of them (2008), Int. J. Food Microbiol., 125, 188-196.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.40 | dithiothreitol | 5 mM, 5% residual activity | Pseudomonas fluorescens |  |
| 3.4.24.40 | EDTA | 5 mM, 23% residual activity | Pseudomonas fluorescens | |
| 3.4.24.40 | EGTA | 5 mM, 33% residual activity | Pseudomonas fluorescens | |
| 3.4.24.40 | NaCl | 1 M, 56% residual activity | Pseudomonas fluorescens | |
| 3.4.24.40 | o-phenanthroline | 0.5 mM, 10% residual activity | Pseudomonas fluorescens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.40 | extracellular | - |
Pseudomonas fluorescens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.40 | Ca2+ | required | Pseudomonas fluorescens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.40 | 45000 | - |
x * 45000, SDS-PAGE | Pseudomonas fluorescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.40 | Pseudomonas fluorescens | Q3YAW3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.40 | - |
Pseudomonas fluorescens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.40 | ? | x * 45000, SDS-PAGE | Pseudomonas fluorescens |
| 3.4.24.40 | More | the N-terminal amino acid sequence shares 100% identity with the domain 14-34 of extracellular alkaline endoprotease sequences called AprX | Pseudomonas fluorescens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.40 | AprX | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.40 | 45 | - |
- |
Pseudomonas fluorescens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.40 | 8.5 | - |
- |
Pseudomonas fluorescens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.24.40 | Pseudomonas fluorescens | isoelectric focusing | - |
4 |
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Release 2023.1 (January 2023)
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