Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chi, M.C.; Ong, P.L.; Hsu, W.H.; Chen, Y.H.; Huang, H.B.; Lin, L.L.
    Role of the invariant Asn345 and Asn435 residues in a leucine aminopeptidase from Bacillus kaustophilus as evaluated by site-directed mutagenesis (2008), Int. J. Biol. Macromol., 43, 481-487.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 expressed in Escherichia coli M15 cells Geobacillus kaustophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.1 N345L the mutation results in 93% reduction in LAP activity Geobacillus kaustophilus
3.4.11.1 N345Q the mutation results in 72% reduction in LAP activity Geobacillus kaustophilus
3.4.11.1 N435E the mutation results in complete loss of the LAP activity Geobacillus kaustophilus
3.4.11.1 N435L the mutation results in complete loss of the LAP activity Geobacillus kaustophilus
3.4.11.1 N435Q the mutation results in complete loss of the LAP activity Geobacillus kaustophilus
3.4.11.1 N435R the mutation results in complete loss of the LAP activity Geobacillus kaustophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.23
-
 Leu-p-nitroanilide wild type enzyme, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2 Geobacillus kaustophilus
3.4.11.1 0.52
-
 Leu-p-nitroanilide mutant enzyme N345Q, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2 Geobacillus kaustophilus
3.4.11.1 0.97
-
 Leu-p-nitroanilide mutant enzyme N345L, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2 Geobacillus kaustophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Zn2+ LAP contains two Zn2+ ions in the active site Geobacillus kaustophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 54000
-
 SDS-PAGE Geobacillus kaustophilus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Geobacillus kaustophilus Q7X2C8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 Ni2+-NTA resin column chromatography Geobacillus kaustophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 11.4
-
 mutant enzyme N345L, using Leu-p-nitroanilide as substrate, at pH 8.0 Geobacillus kaustophilus
3.4.11.1 28.8
-
 mutant enzyme N345Q, using Leu-p-nitroanilide as substrate, at pH 8.0 Geobacillus kaustophilus
3.4.11.1 160
-
 wild type enzyme, using Leu-p-nitroanilide as substrate, at pH 8.0 Geobacillus kaustophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 Leu-4-nitroanilide + H2O
-
 Geobacillus kaustophilus Leu + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
 Geobacillus kaustophilus
3.4.11.1 leucine aminopeptidase
-
 Geobacillus kaustophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 63
-
 the melting temperature of the wild type enzyme is at 63°C Geobacillus kaustophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 0.17
-
 Leu-p-nitroanilide mutant enzyme N345L, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2 Geobacillus kaustophilus
3.4.11.1 0.63
-
 Leu-p-nitroanilide mutant enzyme N345Q, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2 Geobacillus kaustophilus
3.4.11.1 5.92
-
 Leu-p-nitroanilide wild type enzyme, in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM NiCl2 Geobacillus kaustophilus