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Literature summary extracted from

  • Zhang, Y.; Lu, N.; Gao, Z.
    Hemin-H2O2-NO2(-) induced protein oxidation and tyrosine nitration are different from those of SIN-1: a study on glutamate dehydrogenase nitrative/oxidative modification (2009), Int. J. Biochem. Cell Biol., 41, 907-915.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.1.2 nitrosylation results reveal both hemin-H2O2-NO2 and 3-morpholinosydnonimine hydrochloride can cause inactivation of GDH through protein oxidation and tyrosine nitration, the impact of the effect of protein oxidation (not thiol oxidation) on enzyme activity is stronger than that of protein tyrosine nitration. Mass spectrometric analysis indicate that nitrated tyrosine residues by hemin-H2O2-NO2 are Tyr262 and Tyr471 while by 3-morpholinosydnonimine hydrochloride are Tyr401 and Tyr493 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.2 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 2-oxoglutarate + NADH + NH3
-
 Bos taurus L-glutamate + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 GDH
-
 Bos taurus
1.4.1.2 glutamate dehydrogenase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.2 25
-
 assay at Bos taurus