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Literature summary extracted from

  • Dittmer, N.T.; Gorman, M.J.; Kanost, M.R.
    Characterization of endogenous and recombinant forms of laccase-2, a multicopper oxidase from the tobacco hornworm, Manduca sexta (2009), Insect Biochem. Mol. Biol., 39, 596-606.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression of full-length and amino-terminally truncated laccase-2, both showing lower activity than the native enzyme Manduca sexta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information the enzyme shows typical Michaelis–Menten kinetics, overview Manduca sexta
1.10.3.2 0.43
-
 N-acetyldopamine pH 5.0-5.5, recombinant N-terminally truncated enzyme Manduca sexta
1.10.3.2 0.46
-
 N-acetyldopamine pH 5.0-5.5, recombinant full-length enzyme Manduca sexta
1.10.3.2 0.47
-
 N-beta-alanyldopamine pH 5.0-5.5, native enzyme Manduca sexta
1.10.3.2 0.63
-
 N-acetyldopamine pH 5.0-5.5, native enzyme Manduca sexta
1.10.3.2 1.9
-
 N-beta-alanyldopamine pH 5.0-5.5, recombinant full-length enzyme Manduca sexta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ a multicopper oxidase Manduca sexta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 70000
-
 x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE Manduca sexta
1.10.3.2 90000
-
 x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE Manduca sexta

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Manduca sexta Q8I8Y0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein
-
 Manduca sexta
1.10.3.2 additional information laccase-2 is expressed in an active form rather than as a zymogen Manduca sexta

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme from the pharate pupal cuticle to homogeneity Manduca sexta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 additional information laccase-2 is expressed in an active form rather than as a zymogen Manduca sexta
-
1.10.3.2 pharate pupal cuticle
-
 Manduca sexta
-
1.10.3.2 pupal wing cuticle
-
 Manduca sexta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 0.017
-
 native enzyme in pharate pupa and pupal wing Manduca sexta
1.10.3.2 0.41
-
 purified native enzyme, substrate syringaldazine Manduca sexta
1.10.3.2 4.02
-
 purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Manduca sexta
1.10.3.2 4.18
-
 purified native enzyme, substrate N-beta-alanyldopamine-alanyldopamine Manduca sexta
1.10.3.2 5.01
-
 purified native enzyme, substrate N-acetyldopamine Manduca sexta
1.10.3.2 10.06
-
 purified native enzyme, substrate hydrochinone Manduca sexta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Manduca sexta ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Manduca sexta ?
-
 ?
1.10.3.2 additional information no activity with L-tyrosine Manduca sexta ?
-
 ?
1.10.3.2 N-acetyldopamine + O2
-
 Manduca sexta ?
-
 ?
1.10.3.2 N-beta-alanyldopamine + O2
-
 Manduca sexta ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Manduca sexta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE Manduca sexta
1.10.3.2 More peptide mass fingerprinting analysis, mass spectrometry, overview Manduca sexta

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 laccase-2
-
 Manduca sexta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 1.33
-
 N-acetyldopamine pH 5.0-5.5, recombinant N-terminally truncated enzyme Manduca sexta
1.10.3.2 1.67
-
 N-acetyldopamine pH 5.0-5.5, recombinant full-length enzyme Manduca sexta
1.10.3.2 3
-
 N-beta-alanyldopamine pH 5.0-5.5, native enzyme Manduca sexta
1.10.3.2 3.33
-
 N-beta-alanyldopamine pH 5.0-5.5, recombinant full-length enzyme Manduca sexta
1.10.3.2 4.83
-
 N-acetyldopamine pH 5.0-5.5, native enzyme Manduca sexta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5 5.5
-
 Manduca sexta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 4 8 pH profiles of native and recombinant enzymes, overview Manduca sexta

General Information

EC Number General Information Comment Organism
1.10.3.2 physiological function the enzyme is involved in cuticle sclerotization Manduca sexta