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Literature summary extracted from

  • Susla, M.; Novotny, C.; Erbanova, P.; Svobodova, K.
    Implication of Dichomitus squalens manganese-dependent peroxidase in dye decolorization and cooperation of the enzyme with laccase (2008), Folia Microbiol. (Praha), 53, 479-485.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.13 additional information
-
 2,6-dimethoxyphenol apparent Km, 216 microM/l, chromatographic MnP form 2 Dichomitus squalens
1.11.1.13 additional information
-
 Mn2+ apparent Km, 24 microM/l, chromatographic MnP form 1 Dichomitus squalens
1.11.1.13 additional information
-
 2,6-dimethoxyphenol apparent Km, 417 microM/l, chromatographic MnP form 1 Dichomitus squalens
1.11.1.13 additional information
-
 Mn2+ apparent Km, 44 microM/l, chromatographic MnP form 2 Dichomitus squalens
1.11.1.13 additional information
-
 Mn2+ apparent Km, 47 microM/l, chromatographic MnP form 3 Dichomitus squalens
1.11.1.13 additional information
-
 2,6-dimethoxyphenol apparent Km, 516 microM/l, chromatographic MnP form 3 Dichomitus squalens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.13 Manganese
-
 Dichomitus squalens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.13 50000
-
 determined by SDS-PAGE, chromatographic MnP form 1, 2 and 3 Dichomitus squalens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 Dichomitus squalens
-
 Mn3+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Dichomitus squalens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13 extract is loaded onto a HiPREP26/10 desalting column followd by chromatography on a DEAE-Sepharose and a MonoQ HR5/5 column, finally the active fractions are applied to CIM QA Disks Dichomitus squalens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.13 185
-
 purification step crude medium Dichomitus squalens
1.11.1.13 733
-
 purification step DEAE Sepharose Dichomitus squalens
1.11.1.13 870
-
 purification step Mono Q1 Dichomitus squalens
1.11.1.13 1230
-
 purification step CIM QA disk 3, chromatographic MnP form 3, CIM3 Dichomitus squalens
1.11.1.13 2250
-
 purification step Mono Q2 Dichomitus squalens
1.11.1.13 2480
-
 purification step CIM QA disk 1, chromatographic MnP form 1, CIM1 Dichomitus squalens
1.11.1.13 3430
-
 purification step CIM QA disk 2, chromatographic MnP form 2, CIM2 Dichomitus squalens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 2,6-dimethoxyphenol + H2O2
-
 Dichomitus squalens ?
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Dichomitus squalens Mn3+ + H2O
-
 ?
1.11.1.13 additional information dye decolorization Dichomitus squalens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.13 manganese-dependent peroxidase
-
 Dichomitus squalens
1.11.1.13 MnP
-
 Dichomitus squalens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.13 4
-
 pH optimum for Mn2+ oxidation, chromatographic MnP form 1, 2 and 3 Dichomitus squalens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.13 Dichomitus squalens chromatographic MnP form 1 and 2
-
 3.5