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Literature summary extracted from

  • Lushchak, V.I.; Husak, V.V.; Storey, J.M.; Storey, K.B.
    AMP-deaminase from goldfish white muscle: regulatory properties and redistribution under exposure to high environmental oxygen level (2008), Fish Physiol. Biochem., 35, 443-452.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.6 ADP strong activator, 1 mM ADP activates the enzyme by 3.2fold Carassius auratus
3.5.4.6 ATP strong activator, 1 mM ATP activates the enzyme by 1.7fold Carassius auratus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.6 Ca2+ 25% inhibition at 10 mM Carassius auratus
3.5.4.6 fluoride inhibits AMPD by about 66% at 8 mM Carassius auratus
3.5.4.6 K+ inhibitory at concentrations higher than 80 mM Carassius auratus
3.5.4.6 Mg2+ 15% inhibition at 10 mM Carassius auratus
3.5.4.6 Na+ inhibitory at concentrations higher than 80 mM Carassius auratus
3.5.4.6 phosphate inhibits AMPD by about 66% at 8 mM Carassius auratus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.6 K+ activates AMPD at low concentrations, with maximal activation (1.9fold) at about 80 mM Carassius auratus
3.5.4.6 Na+ activates AMPD at low concentrations, with maximal activation (1.3fold) at about 80 mM Carassius auratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.6 55000
-
 the SDS-PAGE shows that the enzyme is not electrophoretically homogenous, with two bands present at a molecular mass between about 55000 and 60000 Da Carassius auratus
3.5.4.6 60000
-
 the SDS-PAGE shows that the enzyme is not electrophoretically homogenous, with two bands present at a molecular mass between about 55000 and 60000 Da Carassius auratus
3.5.4.6 160000
-
 myosin-bound enzyme, SDS-PAGE Carassius auratus

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.6 Carassius auratus
-
 goldfish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.6 phosphocellulose column chromatography Carassius auratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.6 brain
-
 Carassius auratus
-
3.5.4.6 skeletal muscle white skeletal muscle Carassius auratus
-

Storage Stability

EC Number Storage Stability Organism
3.5.4.6 2-4°C, following the purification process when stored in 1 M KCl, 1 month, the enzyme is highly stable showing virtually no change in activity Carassius auratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.6 AMP + H2O
-
 Carassius auratus IMP + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.6 AMP-aminohydrolase
-
 Carassius auratus
3.5.4.6 AMP-deaminase
-
 Carassius auratus
3.5.4.6 AmpD
-
 Carassius auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.6 6.5
-
-
 Carassius auratus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.6 5.5 7.5 at pH values of 5.5, 7.0 and 7.5, AMPD shows 56, 80 and 54% of its optimal activity, respectively Carassius auratus