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Literature summary extracted from
- Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase (2007), FEBS Lett., 581, 4551-4556.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.13.12.5 | additional information | visualize proteins being secreted by exocytosis | Gaussia princeps |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.12.5 | expression in CHO-K1 cells and PC12D cells | Gaussia princeps |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.13.12.5 | DBHsp-GLuc | dopamine beta-hydroxlase fused to GLase, lower luminescence activity than wild-type GLuc | Gaussia princeps |
| 1.13.12.5 | deltaSP-GLuc | GLuc without signal peptide sequence, no significant luminescence | Gaussia princeps |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.5 | 2-mercaptoethanol | reducing agent | Gaussia princeps |  |
| 1.13.12.5 | dithiothreitol | reducing agent | Gaussia princeps | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.13.12.5 | plasma membrane | - |
Gaussia princeps | 5886 | - |
| 1.13.12.5 | secretory vesicle | - |
Gaussia princeps | 99503 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.5 | Gaussia princeps | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.12.5 | - |
Gaussia princeps |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.5 | coelenterazine + O2 | assay at pH 7.4, 25-30°C, 10 min | Gaussia princeps | coelenteramide + CO2 + hnu | - |
? | |
| 1.13.12.5 | luciferin + O2 | assay at pH 7.4, 25-30°C, 10 min | Gaussia princeps | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.12.5 | Gaussia luciferase | - |
Gaussia princeps |
| 1.13.12.5 | GLase | - |
Gaussia princeps |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.5 | 25 | 30 | assay at | Gaussia princeps |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.5 | 7.4 | - |
assay at | Gaussia princeps |
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Release 2023.1 (January 2023)
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