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Literature summary extracted from

  • Rye, C.A.; Isupov, M.N.; Lebedev, A.A.; Littlechild, J.A.
    Biochemical and structural studies of a L-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii (2009), Extremophiles, 13, 179-190.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 expressed in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.2 additional information no inhibition with up to 100 mM of D/L-lactate or pyruvate Sulfurisphaera tokodaii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.8.1.2 acetonitrile in acetonitrile the activity is reduced by over 70% for any concentration tested Sulfurisphaera tokodaii
3.8.1.2 DMSO the enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% (v/v) DMSO, at higher concentrations of DMSO the activity is reduced to below 40% of normal Sulfurisphaera tokodaii
3.8.1.2 Ethanol in 10% (v/v) ethanol activity is still 40% of normal, further increases in ethanol concentration reduce the activity until at 50% (v/v) ethanol less than 10% activity is observed Sulfurisphaera tokodaii
3.8.1.2 Methanol the activity in 10% (v/v) methanol is only 25% of normal, similar activity is observed for the higher concentrations of methanol Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Sulfurisphaera tokodaii Q96XE7 201 amino acids long hypothetical 2-haloalkanoic acid dehalogenase
-
3.8.1.2 Sulfurisphaera tokodaii 7 Q96XE7 201 amino acids long hypothetical 2-haloalkanoic acid dehalogenase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2 Ni-NTA column chromatography and gel filtration Sulfurisphaera tokodaii

Subunits

EC Number Subunits Comment Organism
3.8.1.2 homodimer x-ray crystallography Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
3.8.1.2 L-haloacid dehalogenase
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.2 60
-
-
 Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.2 50 90 the enzyme is stable to prolonged exposure to 50°C, losing only 8% activity after 90 min incubation, as the temperature is increased the stability decreases, the enzyme is thermostable with a half-life of over 1 h at 70°C Sulfurisphaera tokodaii