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Literature summary extracted from

  • Berger, F.; Paulmurugan, R.; Bhaumik, S.; Gambhir, S.S.
    Uptake kinetics and biodistribution of 14C-D-luciferin--a radiolabeled substrate for the firefly luciferase catalyzed bioluminescence reaction: impact on bioluminescence based reporter gene imaging (2008), Eur. J. Nucl. Med. Mol. Imaging, 35, 2275-2285.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.12.7 analysis optical bioluminescence-based molecular imaging Photinus pyralis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 expression in HEK-293T cells Photinus pyralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.7 Mg2+ required Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 D-luciferin + ATP + O2
-
 Photinus pyralis oxyluciferin + CO2 + AMP + hv + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
 Photinus pyralis
1.13.12.7 fluc
-
 Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
 assay at Photinus pyralis