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Literature summary extracted from

  • Shao, X.; Gao, Y.; Jiang, M.; Li, L.
    Deletion and site-directed mutagenesis of laccase from Shigella dysenteriae results in enhanced enzymatic activity and thermostability (2009), Enzyme Microb. Technol., 44, 274-280.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression in Escherichia coli as soluble intein fusion protein Shigella dysenteriae

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.2 E106F site-directed mutagenesis with additional deletion of residues L351-G378, the mutant WlacD shows 3.5fold increased activity and enhanced thermal stability compared to the wild-type enzyme Shigella dysenteriae
1.10.3.2 E106F site-directed mutagenesis, the mutant WlacS shows 2.2fold increased activity compared to the wild-type enzyme Shigella dysenteriae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 EDTA completely inhibited by 200 mM Shigella dysenteriae
1.10.3.2 NaN3 completely inhibited by 50 mM Shigella dysenteriae
1.10.3.2 Pb2+ inhibits 50% at 10 mM Shigella dysenteriae
1.10.3.2 SDS completely inhibited by 32 mM Shigella dysenteriae
1.10.3.2 thiolglycolic acid completely inhibited by 60 mM Shigella dysenteriae
1.10.3.2 Zn2+ inhibits 40% at 10 mM Shigella dysenteriae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.086
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.0, 25°C, recombinant wild-type enzyme Shigella dysenteriae
1.10.3.2 0.112
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.0, 25°C, recombinant mutant WlacS Shigella dysenteriae
1.10.3.2 0.129
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.0, 25°C, recombinant mutant WlacD Shigella dysenteriae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ stimulates the wild-type enzyme by 6% at 10 mM, 14.5% at 100 mM, required for activity Shigella dysenteriae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 107000
-
 recombinant mutant WlacD Shigella dysenteriae
1.10.3.2 110000
-
 recombinant wild-type enzyme and mutant WlacS Shigella dysenteriae

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Shigella dysenteriae
-
 strain W202, a clinically isolated wild-type strain
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 24.4
-
 purified recombinant wild-type Wlac in presence of 100 mM Cu2+ Shigella dysenteriae
1.10.3.2 52.9
-
 purified recombinant mutantWlacS in presence of 100 mM Cu2+ Shigella dysenteriae
1.10.3.2 85.1
-
 purified recombinant mutantWlacD in presence of 100 mM Cu2+ Shigella dysenteriae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Shigella dysenteriae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Wlac
-
 Shigella dysenteriae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 25
-
-
 Shigella dysenteriae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2
-
 25 purified recombinant enzyme, stable Shigella dysenteriae
1.10.3.2 35
-
 purified recombinant enzyme, 2 h, loss of 30% activity Shigella dysenteriae
1.10.3.2 45
-
 purified recombinant enzyme, 1 h, loss of 30% activity Shigella dysenteriae
1.10.3.2 75
-
 purified recombinant enzyme, 5 min, loss of 90% activity Shigella dysenteriae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 18.2
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.0, 25°C, recombinant wild-type enzyme Shigella dysenteriae
1.10.3.2 36.5
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.0, 25°C, recombinant mutant WlacS Shigella dysenteriae
1.10.3.2 50.9
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.0, 25°C, recombinant mutant WlacD Shigella dysenteriae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.5
-
-
 Shigella dysenteriae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 0.5 8 activity range Shigella dysenteriae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3
-
 4-25°C, 24 h, loss of 40% activity Shigella dysenteriae
1.10.3.2 7
-
 4-25°C, 24 h, stable Shigella dysenteriae
1.10.3.2 8
-
 4°C, 24 h, loss of 25% activity, at 25°C oss of 55% activity Shigella dysenteriae