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Literature summary extracted from

  • Chen, L.; Maloney, K.; Krol, E.; Zhu, B.; Yang, J.
    Cloning, overexpression, purification, and characterization of the maleylacetate reductase from Sphingobium chlorophenolicum strain ATCC 53874 (2009), Curr. Microbiol., 58, 599-603.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.32 biotechnology Sphingobium chlorophenolicum has assembled new catabolic pathways to degrade pentachlorophenol and use the ring-cleavage products as their carbon sources Sphingobium chlorophenolicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32 overexpression in Escherichia coli BL21-AI cells Sphingobium chlorophenolicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.32 0.09
-
 maleylacetate at pH 7.0 Sphingobium chlorophenolicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 36000
-
 recombinant enzyme, determined by SDS-PAGE Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-chloromaleylacetate + NADH Sphingobium chlorophenolicum final enzyme in the pentachlorophenol biodegradation pathway which catalyzes the reductive dehalogenation of 2-chloromaleylacetate to maleylacetate and the subsequent reduction of malyelacetate to 3-oxoadipate maleylacetate + NAD+ + Cl-
-
 ?
1.3.1.32 maleylacetate + NADH Sphingobium chlorophenolicum
-
 3-oxoadipate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Sphingobium chlorophenolicum Q8KN40 strain ATCC 53874
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.32 recombinant poly-His tagged PcpE is purified to higher than 95% purity using affinity chromatography with a Ni-NTA agarose column Sphingobium chlorophenolicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-chloromaleylacetate + NADH final enzyme in the pentachlorophenol biodegradation pathway which catalyzes the reductive dehalogenation of 2-chloromaleylacetate to maleylacetate and the subsequent reduction of malyelacetate to 3-oxoadipate Sphingobium chlorophenolicum maleylacetate + NAD+ + Cl-
-
 ?
1.3.1.32 maleylacetate + NADH
-
 Sphingobium chlorophenolicum 3-oxoadipate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.32 maleylacetate reductase
-
 Sphingobium chlorophenolicum
1.3.1.32 PcpE
-
 Sphingobium chlorophenolicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.32 1.2
-
 maleylacetate at pH 7.0 in 20 mM Tris-HCl Sphingobium chlorophenolicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 7
-
 in Tris-HCl Sphingobium chlorophenolicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.32 5.5
-
 PcpE loses approximately 60% of its maximal activity Sphingobium chlorophenolicum
1.3.1.32 8
-
 PcpE loses approximately 60% of its maximal activity Sphingobium chlorophenolicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Sphingobium chlorophenolicum