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Literature summary extracted from

  • Lee, C.C.; Kibblewhite-Accinelli, R.E.; Smith, M.R.; Wagschal, K.; Orts, W.J.; Wong, D.W.
    Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme (2008), Curr. Microbiol., 57, 301-305.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 Ca2+ stimulates activity with increasing amounts from 5 mM (16%) to 20 mM (31%) Bacillus licheniformis
3.2.1.8 Co2+ stimulates activity from 5 mM (16%) to 10 mM (26%) Bacillus licheniformis
3.2.1.8 Cu2+ stimulates activity at 5 mM by 20% Bacillus licheniformis
3.2.1.8 Li+ stimulates activity with increasing amounts from 5 mM (25%) to 20 mM (38%) Bacillus licheniformis
3.2.1.8 Mg2+ stimulates activity with increasing amounts from 5 mM (14%) to 20 mM (57%) Bacillus licheniformis
3.2.1.8 Mn2+ stimulates activity from 5 mM (27%) to 10 mM (40%) Bacillus licheniformis
3.2.1.8 additional information Fe2+ has little effect on enzyme activity from 5 mM to 10 mM Bacillus licheniformis
3.2.1.8 Zn2+ stimulates activity at 5 mM by 25% Bacillus licheniformis

Application

EC Number Application Comment Organism
3.2.1.8 industry the MS514-Xyn11 enzyme can be useful as an additive to mixtures designed to hydrolyze biomass as well as in applications for which it is desirable to maintain cellulose structure Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expressed from vector pET29-MS14-X11 in Escherichia coli strain BL21(DE3)pLysE Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+ inhibits activity from 10 mM (72%) to 20 mM (88%) Bacillus licheniformis
3.2.1.8 Fe2+ inhibits activity at 20 mM by 27% Bacillus licheniformis
3.2.1.8 Mn2+ inhibits activity at 20 mM by 25% Bacillus licheniformis
3.2.1.8 Zn2+ inhibits activity from 10 mM (7%) to 20 mM (21%) Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 23400
-
 sequence analysis Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Bacillus licheniformis B5SYI8
-
-
3.2.1.8 Bacillus licheniformis MS5-14 B5SYI8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 to apparent homogeneity by gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O highest activity against birchwood xylan Bacillus licheniformis ?
-
 ?
3.2.1.8 birchwood xylan + H2O highest activity against birchwood xylan Bacillus licheniformis MS5-14 ?
-
 ?
3.2.1.8 additional information has negligible activity against insoluble and soluble cellulose substrates (avicel and carboxymethyl cellulose, respectively) and other glucose-based substrates (laminarin and lichenan) Bacillus licheniformis ?
-
 ?
3.2.1.8 additional information has negligible activity against insoluble and soluble cellulose substrates (avicel and carboxymethyl cellulose, respectively) and other glucose-based substrates (laminarin and lichenan) Bacillus licheniformis MS5-14 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Bacillus licheniformis MS5-14 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Bacillus licheniformis
3.2.1.8 Xyn11
-
 Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 40 50
-
 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 is stable at temperatures up to 50°C. At 55°C, more than half of the activity is lost, and no activity remains at 60°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 7
-
 Bacillus licheniformis