EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.11 | additional information | nuclear polyphosphates decrease and activity of exopolyphosphatase increases after embryo cellularization until the end of embryogenesis | Rhipicephalus microplus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.11 | heparin | heparin (0.02 mg/ml) inhibits nuclear and mitochondrial PPX activity in about 90 and 95% | Rhipicephalus microplus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.11 | 0.0002 | - |
(phosphate)3 | mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 | Rhipicephalus microplus | |
3.6.1.11 | 0.0007 | - |
(phosphate)3 | nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 | Rhipicephalus microplus | |
3.6.1.11 | 0.0009 | - |
(phosphate)65 | nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 | Rhipicephalus microplus | |
3.6.1.11 | 0.0011 | - |
(phosphate)15 | nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 | Rhipicephalus microplus | |
3.6.1.11 | 0.0028 | - |
(phosphate)15 | mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 | Rhipicephalus microplus | |
3.6.1.11 | 0.0036 | - |
(phosphate)65 | mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5 | Rhipicephalus microplus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.11 | mitochondrion | - |
Rhipicephalus microplus | 5739 | - |
3.6.1.11 | additional information | existence of 2 different PPX isoforms operating in the nuclei and mitochondria with distinct metal dependence, inhibitor and activator sensitivities | Rhipicephalus microplus | - |
- |
3.6.1.11 | nucleus | - |
Rhipicephalus microplus | 5634 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.11 | Co2+ | 0.1 mM Co2+, PPX activity is stimulated by a factor of two in the nuclear fraction, but not in the mitochondrial fraction | Rhipicephalus microplus | |
3.6.1.11 | Mg2+ | 2.5 mM Mg2+, PPX activity is stimulated by a factor of two in the nuclear fraction and in the mitochondrial fraction | Rhipicephalus microplus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.11 | (phosphate)n + H2O | Rhipicephalus microplus | regulatory enzyme in polyphosphate metabolism | (phosphate)n-1 + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.11 | Rhipicephalus microplus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.1.11 | egg | segmentation stage, 9th day after oviposition | Rhipicephalus microplus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.11 | additional information | - |
increasing amounts of total RNA extracted from eggs progressively enhance nuclear PPX activity, whereas it exerts no effect on mitochondrial PPX activity, nuclear PPX activity increases throughout embryogenesis | Rhipicephalus microplus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.11 | (phosphate)15 + H2O | - |
Rhipicephalus microplus | (phosphate)14 + phosphate | - |
? | |
3.6.1.11 | (phosphate)65 + H2O | - |
Rhipicephalus microplus | (phosphate)64 + phosphate | - |
? | |
3.6.1.11 | (phosphate)n + H2O | regulatory enzyme in polyphosphate metabolism | Rhipicephalus microplus | (phosphate)n-1 + phosphate | - |
? | |
3.6.1.11 | triphosphate + H2O | - |
Rhipicephalus microplus | diphosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.11 | exopolyphosphatase | - |
Rhipicephalus microplus |
3.6.1.11 | polyphosphate-phosphohydrolase | - |
Rhipicephalus microplus |
3.6.1.11 | PPX | - |
Rhipicephalus microplus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.11 | 30 | - |
assay at | Rhipicephalus microplus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.11 | 7.5 | - |
assay at | Rhipicephalus microplus |