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Literature summary extracted from

  • Alves, R.M.; Antonucci, G.A.; Paiva, H.H.; Cintra, A.C.; Franco, J.J.; Mendonca-Franqueiro, E.P.; Dorta, D.J.; Giglio, J.R.; Rosa, J.C.; Fuly, A.L.; Dias-Baruffi, M.; Soares, A.M.; Sampaio, S.V.
    Evidence of caspase-mediated apoptosis induced by l-amino acid oxidase isolated from Bothrops atrox snake venom (2008), Comp. Biochem. Physiol. A, 151, 542-550.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.2 extracellular venom Bothrops atrox
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 67000
-
 x * 67000, SDS-PAGE Bothrops atrox

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bothrops atrox
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.2 glycoprotein
-
 Bothrops atrox

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 native enzyme 54fold from venom by gel filtration and ultrafiltration, anion exchange chromatography, and lectin affinity chromatography to homogeneity Bothrops atrox

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Bothrops atrox
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.2 87.5
-
 purified enzyme, substrate L-Leu Bothrops atrox

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-Ile + H2O + O2
-
 Bothrops atrox 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Leu + H2O + O2
-
 Bothrops atrox 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2 best substrate Bothrops atrox 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2
-
 Bothrops atrox phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Trp + H2O + O2
-
 Bothrops atrox indol-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Tyr + H2O + O2
-
 Bothrops atrox 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 additional information substrate specificity, overview Bothrops atrox ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 ? x * 67000, SDS-PAGE Bothrops atrox
1.4.3.2 More peptide mapping Bothrops atrox

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAAO
-
 Bothrops atrox

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 7.2
-
 assay at Bothrops atrox

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Bothrops atrox isoelectric focussing
-
 4.4

General Information

EC Number General Information Comment Organism
1.4.3.2 physiological function the enzyme induces in vitro platelet aggregation, which may be due to H2O2 production, the addition of catalase completely inhibits the aggregation effect. It also shows cytotoxicity towards several cancer cell lines: HL60, Jurkat, B16F10 and PC12 causing apoptosis. The cytotoxicity activity is abolished by catalase Bothrops atrox