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Literature summary extracted from

  • Masayama, A.; Tsukada, K.; Ikeda, C.; Nakano, H.; Iwasaki, Y.
    Isolation of phospholipase D mutants having phosphatidylinositol-synthesizing activity with positional specificity on myo-inositol (2009), ChemBioChem, 10, 559-564.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 mutants are expressed in Escherichia coli Streptomyces antibioticus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.4 additional information the 187H/191Y/385R mutant generates 1-phosphatidylinositol more than 3-phosphatidylinositol, whereas 187T/191Y/385R generates 1-phosphatidylinositol less than 3-phosphatidylinositol Streptomyces antibioticus
3.1.4.4 Y385R the mutation contributes to the selectivity for the 1(3)-PI synthesis Streptomyces antibioticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.0015
-
 phosphatidylcholine wild type enzyme, in sodium acetate buffer (50 mM, pH 5.6), at 37°C Streptomyces antibioticus
3.1.4.4 0.0043
-
 phosphatidylcholine mutant enzyme 187H/191Y/385R, in sodium acetate buffer (50 mM, pH 5.6), at 37°C Streptomyces antibioticus
3.1.4.4 0.0043
-
 phosphatidylcholine mutant enzyme 187T/191Y/385R, in sodium acetate buffer (50 mM, pH 5.6), at 37°C Streptomyces antibioticus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces antibioticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 dioleoyl-phosphatidylcholine + myo-inositol
-
 Streptomyces antibioticus phosphatidylinositol + choline the 187H/191Y/385R mutant generates 1-phosphatidylinositol more than 3-phosphatidylinositol, whereas 187T/191Y/385R generates 1-phosphatidylinositol less than 3-phosphatidylinositol ?
3.1.4.4 phosphatidylcholine + H2O
-
 Streptomyces antibioticus 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phospholipid + H2O
-
 Streptomyces antibioticus phosphatidic acid + alcohol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 phospholipase D
-
 Streptomyces antibioticus
3.1.4.4 PLD
-
 Streptomyces antibioticus