EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.121 | additional information | kcat and Km values are increased by the elevation of temperature, whereas the kcat/Km values are not largely changed | Thermus thermophilus HB8 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.121 | into vector pET-11a and overexpressed in Escherichia coli BL21 (DE3) cells | Thermus thermophilus HB8 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.121 | by oil-microbatch method, at 1.65 A resolution. TAD follows an ordered sequential mechanism in which the coenzyme binds first | Thermus thermophilus HB8 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.121 | D242N | mutation results in a large decrease in thermostability of TAD without dissociation into dimer or monomer | Thermus thermophilus HB8 |
1.1.1.121 | F141A | mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer | Thermus thermophilus HB8 |
1.1.1.121 | F249A | mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer | Thermus thermophilus HB8 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.121 | 1,10-phenanthroline | uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose | Thermus thermophilus HB8 | |
1.1.1.121 | 2,3-benzofuran | uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose | Thermus thermophilus HB8 | |
1.1.1.121 | catechol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | Hexestrol | uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose | Thermus thermophilus HB8 | |
1.1.1.121 | indazole | - |
Thermus thermophilus HB8 | |
1.1.1.121 | indole | uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose | Thermus thermophilus HB8 | |
1.1.1.121 | additional information | EDTA (1 mM) and p-chloromercuriphenylsulfonate and p-chloromercuribenzoate (0.1 mM) have no effect on the activity | Thermus thermophilus HB8 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.121 | 0.012 | - |
isatin | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.14 | - |
1-Acenaphthenol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.17 | - |
(S)-tetralol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.25 | - |
4-chromanol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.65 | - |
(S)-indanol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 1.3 | - |
(R)-indanol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 1.9 | - |
alpha-tetralone | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 2 | - |
phenyl-1,2-propanedione | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 2 | 3 | D-galactosamine | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 2.5 | - |
(R)-tetralol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 3.7 | - |
2-Cyclohexen-1-ol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 5.5 | - |
D-fucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 27 | - |
cis-benzene dihydrodiol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 33 | - |
2-deoxy-D-galactose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 78 | - |
D-galactose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 131 | - |
D-talose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 139 | - |
D-glucosamine | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 162 | - |
L-fucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 178 | - |
6-deoxy-D-glucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 250 | - |
cellobiose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 262 | - |
L-arabinose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 300 | - |
2-deoxy-D-glucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 580 | - |
D-glucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 781 | - |
lactose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 841 | - |
D-xylose | - |
Thermus thermophilus HB8 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.121 | 27000 | - |
4 * 27000, SDS-PAGE | Thermus thermophilus HB8 |
1.1.1.121 | 100000 | - |
gel filtration | Thermus thermophilus HB8 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.121 | Thermus thermophilus HB8 | Q5SLC4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.121 | by sonication, heating at 70°C for 10 min, ion-exchange chromatography, gel filtration and ultrafiltration | Thermus thermophilus HB8 |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.121 | 4°C | Thermus thermophilus HB8 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.121 | (R)-indanol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | (R)-tetralol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | (S)-(+)-1,2,3,4-tetrahydro-1-naphthol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | (S)-(+)-1-indanol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | (S)-indanol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | (S)-tetralol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | 1-acenaphthenol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | 2-cyclohexen-1-ol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | 2-deoxy-D-galactose + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | 2-deoxy-D-glucose + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | 4-chromanol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | 6-deoxy-D-glucose | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | alpha-tetralone + NADH + H+ | - |
Thermus thermophilus HB8 | ? + NAD+ | - |
? | |
1.1.1.121 | cellobiose + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | cis-benzene dihydrodiol + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | D-fucose + NAD+ | - |
Thermus thermophilus HB8 | D-fucono-delta-lactone + NADH + H+ | - |
? | |
1.1.1.121 | D-galactosamine + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | D-galactose + NAD+ | - |
Thermus thermophilus HB8 | D-galactono-gamma-lactone + NADH + H+ | - |
? | |
1.1.1.121 | D-glucosamine + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | D-glucose + NAD+ | - |
Thermus thermophilus HB8 | D-glucono-delta-lactone + NADH + H+ | - |
? | |
1.1.1.121 | D-talose + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | D-xylose + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | glucono-delta-lactone + NADH + H+ | - |
Thermus thermophilus HB8 | ? + NAD+ | - |
? | |
1.1.1.121 | isatin + NADH + H+ | - |
Thermus thermophilus HB8 | ? + NAD+ | - |
? | |
1.1.1.121 | L-arabinose + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | L-fucose + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | lactose + NAD+ | - |
Thermus thermophilus HB8 | ? + NADH + H+ | - |
? | |
1.1.1.121 | additional information | TAD possesses the catalytic triad Ser136, Tyr149, and Lys153. No activity with L-xylose, D-ribose and 2-deoxy-D-ribose. TAD does not oxidize ketoses (D-fructose and D-sorbose), sugar alcohols (D-sorbitol, galactitol, inositol, L-arabitol and xylitol), uronic acids (D-galacturonic acid and D-glucuronic acid), aldonic acids (L-gulonic acid and 2-keto-L-gulonic acid), aromatic alcohols (benzyl alcohol and catechol), aliphatic alcohols (1-nonanol, 1-decanol, 3-hydroxybutyric acid and 2,3-butanediol), and hydroxysteroids (androsterone, testosterone, 20alpha/beta-hydroxyprogesterone, cholic acid, cortisol and corticosterone). No significant activity ketones (4-nitroacetophenone, camphorquinone, 2,3-hexanedione and diacetyl) and aldehydes (4-nitrobenzaldehyde, pyridine-3-aldehyde, 1-nonanal and citral) | Thermus thermophilus HB8 | ? | - |
? | |
1.1.1.121 | phenyl-1,2-propanedione + NADH + H+ | - |
Thermus thermophilus HB8 | ? + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.121 | tetramer | 4 * 27000, SDS-PAGE | Thermus thermophilus HB8 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.121 | TAD | - |
Thermus thermophilus HB8 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.121 | 80 | 85 | - |
Thermus thermophilus HB8 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.121 | 85 | - |
approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95°C | Thermus thermophilus HB8 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.121 | 0.075 | - |
(R)-tetralol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.1067 | - |
L-fucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.167 | - |
(R)-indanol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.3117 | - |
D-galactosamine | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.423 | - |
D-xylose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.6217 | - |
D-glucosamine | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 3.017 | - |
D-talose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 3.5 | - |
2-Cyclohexen-1-ol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 3.85 | - |
D-glucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 3.9 | - |
alpha-tetralone | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 4.55 | - |
L-arabinose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 5.767 | - |
cellobiose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 6.35 | - |
6-deoxy-D-glucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 6.93 | - |
2-deoxy-D-glucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 7.383 | - |
cis-benzene dihydrodiol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 8.25 | - |
D-galactose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 8.73 | - |
D-fucose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 9.23 | - |
lactose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 12.8 | - |
2-deoxy-D-galactose | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 16.83 | - |
phenyl-1,2-propanedione | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 40.3 | - |
4-chromanol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 138.7 | - |
(S)-indanol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 161.7 | - |
(S)-tetralol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 433.3 | - |
1-Acenaphthenol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 981.7 | - |
isatin | - |
Thermus thermophilus HB8 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.121 | 2 | 13 | - |
Thermus thermophilus HB8 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.121 | additional information | TAD does not exhibit dehydrogenase activity with NADP+ as the coenzyme | Thermus thermophilus HB8 | |
1.1.1.121 | NAD+ | - |
Thermus thermophilus HB8 |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.121 | 0.013 | - |
Hexestrol | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.025 | - |
1,10-phenanthroline | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.042 | - |
2,3-benzofuran | - |
Thermus thermophilus HB8 | |
1.1.1.121 | 0.48 | - |
indole | - |
Thermus thermophilus HB8 |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.121 | 0.063 | - |
- |
Thermus thermophilus HB8 | Hexestrol | |
1.1.1.121 | 0.18 | - |
- |
Thermus thermophilus HB8 | 1,10-phenanthroline | |
1.1.1.121 | 0.2 | - |
- |
Thermus thermophilus HB8 | 2,3-benzofuran | |
1.1.1.121 | 0.48 | - |
- |
Thermus thermophilus HB8 | indole | |
1.1.1.121 | 1.2 | - |
- |
Thermus thermophilus HB8 | catechol | |
1.1.1.121 | 1.9 | - |
- |
Thermus thermophilus HB8 | indazole |