EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.11.67 | additional information | transgenic flies expressing inverted repeats of the CG11033 coding region under the influence of the UAS promoter are crossed with act5CGal4 flies. The presence of Gal4 leads to transcription of inverted repeats under the influence of UAS promoter bearing Gal4 binding sites. Flies bearing only the inverted repeats are used as a control. The expression of dsRNA arising out of transcription of inverted repeats brings about downregulation of CG11033. The mutants exhibit multiple nucleoli, which are smaller in size. Quantitative real time PCR analysis shows reduction of dKDM2 transcript level relative to tubulin mRNA level in RNAi knockdown larvae. Analysis of histone modifications in the dKDM2 RNAi knockdown mutants, overview | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.67 | [histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 3 2-oxoglutarate + 3 O2 | Drosophila melanogaster | dKDM2 is a histone lysine demethylase with specificity for H3K4me3, not H3K36me2, and regulates nucleolar organization. H3K4me3 modification is the site of active transcription | [histone H3]-L-lysine4 + 3 succinate + 3 formaldehyde + 3 CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.67 | Drosophila melanogaster | - |
gene CG11033 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.67 | [histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 3 2-oxoglutarate + 3 O2 | - |
Drosophila melanogaster | [histone H3]-L-lysine4 + 3 succinate + 3 formaldehyde + 3 CO2 | - |
? | |
1.14.11.67 | [histone H3]-N6,N6,N6-trimethyl-L-lysine4 + 3 2-oxoglutarate + 3 O2 | dKDM2 is a histone lysine demethylase with specificity for H3K4me3, not H3K36me2, and regulates nucleolar organization. H3K4me3 modification is the site of active transcription | Drosophila melanogaster | [histone H3]-L-lysine4 + 3 succinate + 3 formaldehyde + 3 CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.67 | H3K4me3 demethylase | - |
Drosophila melanogaster |
1.14.11.67 | KDM2 | - |
Drosophila melanogaster |
1.14.11.67 | Lid | - |
Drosophila melanogaster |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.11.67 | malfunction | H3K4me3 is an important epigenetic landmark for active transcription, failure to erase this mark can result in aberrant transcription of rDNA repeats and failure to form a single compact nucleolus | Drosophila melanogaster |
1.14.11.67 | physiological function | dKDM2 plays an important role in nucleolar structure organization | Drosophila melanogaster |