Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jimenez, A.; Santos, M.A.; Revuelta, J.L.
    Phosphoribosyl pyrophosphate synthetase activity affects growth and riboflavin production in Ashbya gossypii (2008), BMC Biotechnol., 8, 67.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.6.1 synthesis the enzyme is useful in industrial production of the vitamin riboflavin using the fungus Ashbya gossypii Eremothecium gossypii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 genes AGR371C and AGL080C, overexpression of wild-type and mutant deregulated isozymes Agr371cp and Agl080cp, phylogenetic tree of the PRS proteins from Ashbya gossypii and Saccharomyces cerevisiae Eremothecium gossypii

Protein Variants

EC Number Protein Variants Comment Organism
2.7.6.1 additional information construction of engineered AGR371C and AGL080C mutant strains, which show increased riboflavin synthesis compared to the wild-type strain Eremothecium gossypii

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Eremothecium gossypii
-
 strain ATCC 10895, two genes AGR371C and AGL080C encode isozymes AgPRS2,4 and AgPRS3, respectively
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 0.01
-
 wild-type strain Eremothecium gossypii
2.7.6.1 0.011 0.012 mutant strain GPD-PRS2,4, engineered gene AGR371C Eremothecium gossypii
2.7.6.1 0.017 0.018 mutant strain GPD-PRS3, engineered gene AGL080C Eremothecium gossypii

Synonyms

EC Number Synonyms Comment Organism
2.7.6.1 PRPP synthetase
-
 Eremothecium gossypii
2.7.6.1 PRS
-
 Eremothecium gossypii