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Literature summary extracted from
- Utilization of the recombinant human beta-carotene-15,15-monooxygenase gene in Escherichia coli and mammalian cells (2008), Biotechnol. Lett., 30, 735-741.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.13.11.63 | analysis | BCMO function in mammalian cells is analyzed by a retinoic acid receptor assay, which responds to the metabolic conversion of beta-carotene to retinoic acid in vivo, this tool can be used to screen more active BCMO for the industrial and pharmacological purpose of retinal production from beta-carotene | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.63 | into a Flag fusion-modified mammalian expression vector pcDNA3 and into the bacterial vectors pET-15b and pGEX-4T2 | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.13.11.63 | T381L | mutant, shows 1.5fold higher catalytic efficiency for beta-carotene and 1.7fold higher retinal production than the wild-type | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.63 | 0.028 | - |
beta-carotene | T381L mutant | Homo sapiens |  |
| 1.13.11.63 | 0.031 | - |
beta-carotene | wild-type | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.63 | 62000 | - |
theoretical | Homo sapiens |
| 1.13.11.63 | 65000 | - |
determined by SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.63 | beta-carotene + O2 | Homo sapiens | - |
retinal | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.63 | Homo sapiens | Q9HAY6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.63 | using a HiTrap Chelating HP column | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.13.11.63 | A-704 cell | human kidney cell line | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.63 | beta-carotene + O2 | - |
Homo sapiens | retinal | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.63 | BCMO | - |
Homo sapiens |
| 1.13.11.63 | beta-carotene-15,15'-monooxygenase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.63 | 37 | - |
activity assay | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.63 | 8 | - |
activity assay | Homo sapiens |
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Release 2023.1 (January 2023)
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