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Literature summary extracted from

  • Salaheddin, C.; Spadiut, O.; Ludwig, R.; Tan, T.C.; Divne, C.; Haltrich, D.; Peterbauer, C.
    Probing active-site residues of pyranose 2-oxidase from Trametes multicolor by semi-rational protein design (2009), Biotechnol. J., 4, 535-543.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 heterologously expressed in Escherichia coli BL21 (DE3) Trametes ochracea

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.10 H548I mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 H548R mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 N593R mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 Q448C mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 Q448N mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 Q448S mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 R472G mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 R472L mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 T169G mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 T169N mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 T169S mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 V546C mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 V546C/T169N mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 V546G mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 V546G/T169G mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 V546P mutant, analysis of kinetic parameters Trametes ochracea
1.1.3.10 V546P/T169G mutant, analysis of kinetic parameters Trametes ochracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.327
-
 D-glucose T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.394
-
 D-glucose T169S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.527
-
 D-glucose N593R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.658
-
 D-glucose V546P/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.691
-
 D-glucose T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.698
-
 D-glucose wild-type, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.773
-
 D-glucose R472G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.8 11 D-glucose H548I, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.88
-
 D-glucose H548R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.886
-
 D-glucose R472L, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.952
-
 D-glucose V546C/T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.983
-
 D-galactose V546P/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.984
-
 D-glucose V546G/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 2.2
-
 D-glucose V546P, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 2.48
-
 D-galactose T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 3.06
-
 D-glucose V546C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 3.56
-
 D-galactose T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 5.7
-
 D-glucose V546G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 6.38
-
 D-galactose R472G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 7.21
-
 D-galactose R472L, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 7.39
-
 D-galactose N593R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 8.09
-
 D-galactose wild-type, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 9.27
-
 D-galactose H548R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 9.89
-
 D-galactose H548I, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 10.4
-
 D-galactose V546C/T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 12.9
-
 D-galactose T169S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 28
-
 D-glucose Q448N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 29.4
-
 D-galactose V546G/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 30
-
 D-glucose Q448S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 46.2
-
 D-galactose V546C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 50.6
-
 D-galactose V546P, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 100
-
 D-glucose Q448C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 200
-
 D-galactose V546G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 750
-
 D-galactose Q448S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 940
-
 D-galactose Q448N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 1260
-
 D-galactose Q448C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Trametes ochracea Q7ZA32
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 Ni2+ immobilized column with Chelating Sepharose Fast Flow combined with Äkta Purifier System, concentrated in an Amicon Ultra Centrifugal Filter Device Trametes ochracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-galactose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-galactose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
-
 Trametes ochracea 2-dehydro-D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 ?
1.1.3.10 D-galactose + O2
-
 Trametes ochracea 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-glucose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-glucose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
-
 Trametes ochracea 2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 ?
1.1.3.10 D-glucose + O2
-
 Trametes ochracea 2-dehydro-D-glucose + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2Ox
-
 Trametes ochracea
1.1.3.10 pyranose 2-Oxidase
-
 Trametes ochracea
1.1.3.10 TmP2Ox
-
 Trametes ochracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 0.119
-
 D-glucose V546G/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.232
-
 D-glucose V546P/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.245
-
 D-galactose V546P/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.262
-
 D-glucose T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.273
-
 D-galactose T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.331
-
 D-galactose V546G/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.356
-
 D-galactose H548R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.433
-
 D-galactose Q448N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.622
-
 D-galactose Q448C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.654
-
 D-galactose V546C/T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 0.963
-
 D-glucose V546C/T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 1.51
-
 D-glucose Q448C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 1.53
-
 D-galactose Q448S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 1.57
-
 D-galactose N593R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 1.6
-
 D-galactose V546P, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 1.69
-
 D-galactose R472L, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 1.96
-
 D-galactose T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 2.07
-
 D-galactose R472G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 2.41
-
 D-glucose T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 2.66
-
 D-galactose H548I, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 2.73
-
 D-galactose wild-type, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 4.51
-
 D-galactose V546G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 4.73
-
 D-glucose Q448N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 5.42
-
 D-glucose Q448S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 5.53
-
 D-galactose T169S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 6.57
-
 D-galactose V546C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 9.2
-
 D-glucose H548R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 15.6
-
 D-glucose N593R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 15.8
-
 D-glucose V546P, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 21.8
-
 D-glucose T169S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 23.6
-
 D-glucose V546G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 30.9
-
 D-glucose R472L, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 31.6
-
 D-glucose H548I, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 32.2
-
 D-glucose R472G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 35.4
-
 D-glucose wild-type, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea
1.1.3.10 88.6
-
 D-glucose V546C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay Trametes ochracea