Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fazary, A.E.; Ju, Y.H.
    Production, partial purification and characterization of feruloyl esterase by Aspergillus awamori in submerged fermentation (2008), Biotechnol. J., 3, 1264-1275.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 biofuel production The biorefining of crop components, such as starch, grain fiber, and crop residues to fermentable substrates for the production of high-value products, such as ethanol and butanol, provides a source of renewable energy Aspergillus awamori
3.1.1.73 food industry release of phenolic acids Aspergillus awamori
3.1.1.73 paper production This enzyme reaction is one of the controlling factors for increasing the extent of degradation of lignocellulosic biomass for bioenergy conversion, and the manufacture of pulp and paper Aspergillus awamori
3.1.1.73 pharmacology release of phenolic acids Aspergillus awamori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Aspergillus awamori
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 31000
-
 SDS-PAGE Aspergillus awamori

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus awamori
-
-
-
3.1.1.73 Aspergillus awamori IFO 4033
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 by centrifugation and dialysis, anion exchange chromatography and ammonium sulfate fractionation, to homogeneity, between 5.7fold and 8.3fold, and with a yield between 30-44% dependent on the carbon source Aspergillus awamori
3.1.1.73 ion exchange chromatography and microfiltration to homogeneity, salting-out using ammonium sulfate Aspergillus awamori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.73 culture supernatant
-
 Aspergillus awamori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 2.19
-
 extracted and filtrated fraction, de-starched corncobs as carbon source Aspergillus awamori
3.1.1.73 2.191
-
 6fold purified enzyme, with de-starched corncobs as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.191
-
 with de-starched corncobs as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.24
-
 extracted and filtrated fraction, de-starched wheat straw as carbon source Aspergillus awamori
3.1.1.73 2.241
-
 7.7fold purified enzyme, with de-starched wheat straw as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.241
-
 with de-starched wheat straw as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.26
-
 extracted and filtrated fraction, wheat germ as carbon source Aspergillus awamori
3.1.1.73 2.262
-
 6.5fold purified enzyme, with wheat germ as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.262
-
 with wheat germ as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.31
-
 extracted and filtrated fraction, de-esterified wheat straw as carbon source Aspergillus awamori
3.1.1.73 2.319
-
 8.3fold purified enzyme, with de-esterified wheat straw as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.319
-
 with de-esterified wheat straw as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.32
-
 extracted and filtrated fraction, corncobs as carbon source Aspergillus awamori
3.1.1.73 2.321
-
 6.1fold purified enzyme, with corncobs as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.321
-
 with corncobs as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.35
-
 extracted and filtrated fraction, wheat straw as carbon source Aspergillus awamori
3.1.1.73 2.35
-
 8.2fold purified enzyme, with wheat straw as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.35
-
 with wheat straw as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.357
-
 5.7fold purified enzyme, with de-esterified corncobs as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.357
-
 with de-esterified corncobs as carbon source, at 30°C, pH 6 Aspergillus awamori
3.1.1.73 2.36
-
 extracted and filtrated fraction, de-esterified corncobs as carbon source Aspergillus awamori
3.1.1.73 2.52
-
 first DEAE-cellulose fraction, de-starched wheat straw as carbon source Aspergillus awamori
3.1.1.73 2.56
-
 first DEAE-cellulose fraction, wheat germ as carbon source Aspergillus awamori
3.1.1.73 2.71
-
 first DEAE-cellulose fraction, wheat straw as carbon source Aspergillus awamori
3.1.1.73 2.82
-
 first DEAE-cellulose fraction, corncobs as carbon source Aspergillus awamori
3.1.1.73 3.1
-
 first DEAE-cellulose fraction, de-esterified wheat straw as carbon source Aspergillus awamori
3.1.1.73 3.33
-
 first DEAE-cellulose fraction, de-esterified corncobs as carbon source Aspergillus awamori
3.1.1.73 3.57
-
 second DEAE-cellulose fraction, de-esterified wheat straw as carbon source Aspergillus awamori
3.1.1.73 3.85
-
 second DEAE-cellulose fraction, wheat straw as carbon source Aspergillus awamori
3.1.1.73 4.15
-
 first DEAE-cellulose fraction, de-starched corncobs as carbon source Aspergillus awamori
3.1.1.73 5
-
 second DEAE-cellulose fraction, wheat germ as carbon source Aspergillus awamori
3.1.1.73 6.92
-
 second DEAE-cellulose fraction, de-starched wheat straw as carbon source Aspergillus awamori
3.1.1.73 8.28
-
 second DEAE-cellulose fraction, corncobs as carbon source Aspergillus awamori
3.1.1.73 8.99
-
 second DEAE-cellulose fraction, de-starched corncobs as carbon source Aspergillus awamori
3.1.1.73 9.82
-
 second DEAE-cellulose fraction, de-esterified corncobs as carbon source Aspergillus awamori
3.1.1.73 13.1
-
 ammonium sulfate fraction, de-starched corncobs as carbon source Aspergillus awamori
3.1.1.73 13.32
-
 ammonium sulfate fraction, de-esterified corncobs as carbon source Aspergillus awamori
3.1.1.73 14.11
-
 ammonium sulfate fraction, corncobs as carbon source Aspergillus awamori
3.1.1.73 14.77
-
 ammonium sulfate fraction, wheat germ as carbon source Aspergillus awamori
3.1.1.73 15.61
-
 ammonium sulfate fraction, de-starched wheat straw as carbon source Aspergillus awamori
3.1.1.73 19.31
-
 ammonium sulfate fraction, de-esterified wheat straw as carbon source Aspergillus awamori
3.1.1.73 19.31
-
 ammonium sulfate fraction, wheat straw as carbon source Aspergillus awamori

Storage Stability

EC Number Storage Stability Organism
3.1.1.73 -20°C, 4 months Aspergillus awamori
3.1.1.73 at room temperature, after 2 days feruloyl esterases enzyme loses about 15% of its initial activity and only about one third of the initial activity remains at the end of 1 week, on refrigeration, no apparent loss of activity is found up to 4 weeks, but after 4 weeks a marginal decrease in activity (5-10%) is found. The enzyme retains almost its full activity when stored deep frozen (-20°C) for up to 4 months. Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ethyl ferulate + H2O
-
 Aspergillus awamori ferulic acid + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Aspergillus awamori IFO 4033 ferulic acid + ethanol
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Aspergillus awamori ethanol + ferulic acid
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Aspergillus awamori IFO 4033 ethanol + ferulic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 31000, SDS-PAGE Aspergillus awamori
3.1.1.73 monomer 1 * 31000 SDS-PAGE Aspergillus awamori

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 feruloyl esterase
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 30
-
-
 Aspergillus awamori
3.1.1.73 55
-
 partially purified enzyme, in 0.1 M MOPS buffer, pH 5.5 Aspergillus awamori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 additional information
-
 growth and feruloyl esterase production almost completely stopped at higher temperature (45°C) Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5.5
-
 partially purified enzyme Aspergillus awamori
3.1.1.73 6
-
-
 Aspergillus awamori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 2 9.5 growth and feruloyl esterase production almost completely stopped at pH 10 Aspergillus awamori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 4 9 at 25°C Aspergillus awamori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Aspergillus awamori isoelectric focusing
-
 5.53
3.1.1.73 Aspergillus awamori isoelectric focusing (IEF)
-
 5.53

Expression

EC Number Organism Comment Expression
3.1.1.73 Aspergillus awamori sodium nitrate is the best nitrogen source. Inorganic nitrogen source yields better feruloyl esterases activity than an organic nitrogen source, such as urea. Moderate to good levels of feruloyl estrerases with ammonium chloride, ammonium citrate, ammonium tartrate and potassium nitrate as nitrogen sources. Optimal conditions for high level of feruloyl esterase production are: culturing temperature 30°C, shaker speed 250 rpm, initial pH 6, with wheat straw, corncobs or wheat germ as carbon source and sodium nitrate or urea as nitrogen source up