BRENDA - Enzyme Database show

A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide

Heumann, S.; Eberl, A.; Fischer-Colbrie, G.; Pobeheim, H.; Kaufmann, F.; Ribitsch, D.; Cavaco-Paulo, A.; Guebitz, G.M.; Biotechnol. Bioeng. 102, 1003-1011 (2009)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.13
1-aminohexane
-
Nocardia farcinica
3.5.1.13
AgNO3
-
Nocardia farcinica
3.5.1.13
CuSO4
-
Nocardia farcinica
3.5.1.13
HgCl2
-
Nocardia farcinica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.13
0.07
-
p-nitroacetanilide
-
Nocardia farcinica
3.5.1.13
5.5
-
hexanoamide
-
Nocardia farcinica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.13
50000
-
4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis
Nocardia farcinica
3.5.1.13
54000
-
4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis
Nocardia farcinica
3.5.1.13
190000
-
gel filtration
Nocardia farcinica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.13
Nocardia farcinica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.13
-
Nocardia farcinica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.13
2.4
-
-
Nocardia farcinica
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.13
-18°C, 1 week, 18% loss of activity
Nocardia farcinica
3.5.1.13
25°C, 1 week, 5% loss of activity
Nocardia farcinica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.13
acetamide + H2O
-
696876
Nocardia farcinica
acetic acid + NH3
-
-
-
?
3.5.1.13
adipic acid bishexylamide + H2O
-
696876
Nocardia farcinica
adipic acid + dihexylamine
-
-
-
?
3.5.1.13
asparagine + H2O
-
696876
Nocardia farcinica
aspartic acid + NH3
-
-
-
?
3.5.1.13
benzamide + H2O
-
696876
Nocardia farcinica
benzoic acid + NH3
-
-
-
?
3.5.1.13
butyramide + H2O
-
696876
Nocardia farcinica
butanoic acid + NH3
-
-
-
?
3.5.1.13
hexanoamide + ?
-
696876
Nocardia farcinica
hexanoic acid + NH3
-
-
-
?
3.5.1.13
mandelamine + H2O
-
696876
Nocardia farcinica
mandelic acid + NH3
-
-
-
?
3.5.1.13
methacrylamide + H2O
-
696876
Nocardia farcinica
methacrylic acid + NH3
-
-
-
?
3.5.1.13
additional information
surface hydrolysis of polyamide 6
696876
Nocardia farcinica
?
-
-
-
-
3.5.1.13
nicotinamide + H2O
-
696876
Nocardia farcinica
nicotinic acid + NH3
-
-
-
?
3.5.1.13
p-nitroacetanilide + H2O
-
696876
Nocardia farcinica
4-nitroaniline + acetic acid
-
-
-
?
3.5.1.13
propionamide + H2O
-
696876
Nocardia farcinica
propanoic acid + NH3
-
-
-
?
3.5.1.13
stearamide + H2O
-
696876
Nocardia farcinica
stearic acid + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.13
tetramer
4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis
Nocardia farcinica
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.13
50
-
pH8
Nocardia farcinica
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.13
50
-
half life time: 35 min
Nocardia farcinica
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.13
3.5
-
hexanoamide
-
Nocardia farcinica
3.5.1.13
5.8
-
p-nitroacetanilide
-
Nocardia farcinica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.13
8
11
-
Nocardia farcinica
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.13
6
11
-
Nocardia farcinica
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.13
0.2
-
AgNO3
-
Nocardia farcinica
3.5.1.13
0.2
-
HgCl2
-
Nocardia farcinica
3.5.1.13
2.7
-
1-aminohexane
-
Nocardia farcinica
3.5.1.13
3.2
-
CuSO4
-
Nocardia farcinica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.13
1-aminohexane
-
Nocardia farcinica
3.5.1.13
AgNO3
-
Nocardia farcinica
3.5.1.13
CuSO4
-
Nocardia farcinica
3.5.1.13
HgCl2
-
Nocardia farcinica
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.13
0.2
-
AgNO3
-
Nocardia farcinica
3.5.1.13
0.2
-
HgCl2
-
Nocardia farcinica
3.5.1.13
2.7
-
1-aminohexane
-
Nocardia farcinica
3.5.1.13
3.2
-
CuSO4
-
Nocardia farcinica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.13
0.07
-
p-nitroacetanilide
-
Nocardia farcinica
3.5.1.13
5.5
-
hexanoamide
-
Nocardia farcinica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.13
50000
-
4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis
Nocardia farcinica
3.5.1.13
54000
-
4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis
Nocardia farcinica
3.5.1.13
190000
-
gel filtration
Nocardia farcinica
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.13
-
Nocardia farcinica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.13
2.4
-
-
Nocardia farcinica
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.13
-18°C, 1 week, 18% loss of activity
Nocardia farcinica
3.5.1.13
25°C, 1 week, 5% loss of activity
Nocardia farcinica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.13
acetamide + H2O
-
696876
Nocardia farcinica
acetic acid + NH3
-
-
-
?
3.5.1.13
adipic acid bishexylamide + H2O
-
696876
Nocardia farcinica
adipic acid + dihexylamine
-
-
-
?
3.5.1.13
asparagine + H2O
-
696876
Nocardia farcinica
aspartic acid + NH3
-
-
-
?
3.5.1.13
benzamide + H2O
-
696876
Nocardia farcinica
benzoic acid + NH3
-
-
-
?
3.5.1.13
butyramide + H2O
-
696876
Nocardia farcinica
butanoic acid + NH3
-
-
-
?
3.5.1.13
hexanoamide + ?
-
696876
Nocardia farcinica
hexanoic acid + NH3
-
-
-
?
3.5.1.13
mandelamine + H2O
-
696876
Nocardia farcinica
mandelic acid + NH3
-
-
-
?
3.5.1.13
methacrylamide + H2O
-
696876
Nocardia farcinica
methacrylic acid + NH3
-
-
-
?
3.5.1.13
additional information
surface hydrolysis of polyamide 6
696876
Nocardia farcinica
?
-
-
-
-
3.5.1.13
nicotinamide + H2O
-
696876
Nocardia farcinica
nicotinic acid + NH3
-
-
-
?
3.5.1.13
p-nitroacetanilide + H2O
-
696876
Nocardia farcinica
4-nitroaniline + acetic acid
-
-
-
?
3.5.1.13
propionamide + H2O
-
696876
Nocardia farcinica
propanoic acid + NH3
-
-
-
?
3.5.1.13
stearamide + H2O
-
696876
Nocardia farcinica
stearic acid + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.13
tetramer
4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis
Nocardia farcinica
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.13
50
-
pH8
Nocardia farcinica
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.13
50
-
half life time: 35 min
Nocardia farcinica
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.13
3.5
-
hexanoamide
-
Nocardia farcinica
3.5.1.13
5.8
-
p-nitroacetanilide
-
Nocardia farcinica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.13
8
11
-
Nocardia farcinica
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.13
6
11
-
Nocardia farcinica