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Literature summary extracted from

  • Kai, G.; Li, L.; Jiang, Y.; Yan, X.; Zhang, Y.; Lu, X.; Liao, P.; Chen, J.
    Molecular cloning, characterization of two tropinone reductases in Anisodus acutangulus and enhancement of tropane alkaloids production in AaTRI-transformed hairy roots (2009), Biotechnol. Appl. Biochem., 54, :177-186.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.206 additional information methyl jasmonate can enhance expression of TRI, with weak effect for TRI. TRI is induced weakly in all tissues Anisodus acutangulus
1.1.1.236 additional information methyl jasmonate can enhance expression of TRII, with strong effect for TRII. TRII transcript is strongly induced, reaching the highest level after 24 h of the treatment in root and stem. TRII transcription level is highest in leaf after 12h treatment Anisodus acutangulus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.206 TRI-transformed hairy root lines. Full-length fragment of TRI cDNA insert constructed into the pBI121 expression cassette in place of GUS gene. The vector pCAMBIA1304+ contains the TRI gene under control of CaMV35S promoter. Disarmed Agrobacterium tumefaciens strain C58C1 harboring both Agrobacterium rhizogenes Ri plasmid pRiA4 and pCAMBIA1304+, containing a single TRI gene, used for plant transformation Anisodus acutangulus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.206 methyl jasmonate inhibits expression of TRI in hairy roots Anisodus acutangulus
1.1.1.236 methyl jasmonate inhibits expression of TRII in hairy roots Anisodus acutangulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.206 29460
-
 sequence analysis Anisodus acutangulus
1.1.1.236 28290
-
 sequence analysis Anisodus acutangulus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.206 Anisodus acutangulus B2L2W8
-
-
1.1.1.236 Anisodus acutangulus B2L2W9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.206 leaf transcript level of TRI is low Anisodus acutangulus
-
1.1.1.206 root transcript level of TRI is low Anisodus acutangulus
-
1.1.1.206 stem transcript level of TRI is low Anisodus acutangulus
-
1.1.1.236 leaf transcript level of TRII is high Anisodus acutangulus
-
1.1.1.236 root transcript level of TRII is high Anisodus acutangulus
-
1.1.1.236 stem transcript level of TRII is high Anisodus acutangulus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.206 additional information significant increase of tropane alkaloids after TRI single gene transformation in Anisodus acutangulus Anisodus acutangulus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.206 TRI
-
 Anisodus acutangulus
1.1.1.206 tropinone reductase
-
 Anisodus acutangulus
1.1.1.236 TRII
-
 Anisodus acutangulus
1.1.1.236 tropinone reductase
-
 Anisodus acutangulus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.206 Anisodus acutangulus sequence analysis
-
 6.45
1.1.1.236 Anisodus acutangulus sequence analysis
-
 5.35