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Literature summary extracted from

  • Fujihara, J.; Yasuda, T.; Kunito, T.; Fujii, Y.; Takatsuka, H.; Moritani, T; Takeshita, H.
    Two N-linked glycosylation sites (Asn18 and Asn106) are both required for full enzymatic activity, thermal stability, and resistance to proteolysis in mammalian deoxyribonuclease I (2008), Biosci. Biotechnol. Biochem., 72, 3197-3205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.1 expression of wild-type and mutants in COS-7 cells Bos taurus
3.1.21.1 expression of wild-type and mutants in COS-7 cells Homo sapiens
3.1.21.1 expression of wild-type and mutants in COS-7 cells Equus caballus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.1 N106Q enzyme activity is lower than that of the wild-type, is unstable to heat and less resistant to trypsin Homo sapiens
3.1.21.1 N106Q enzyme activity is lower than that of the wild-type, is unstable to heat and less resistant to trypsin Equus caballus
3.1.21.1 N106Q enzyme activity is lower than that of the wild-type, is unstable to heat, trypsin resistance is similar to that of the wild-type Bos taurus
3.1.21.1 N18Q enzyme activity is lower than that of the wild-type, is unstable to heat and less resistant to trypsin Homo sapiens
3.1.21.1 N18Q enzyme activity is lower than that of the wild-type, is unstable to heat and less resistant to trypsin Equus caballus
3.1.21.1 N18Q enzyme activity is lower than that of the wild-type, is unstable to heat, trypsin resistance is similar to that of the wild-type Bos taurus
3.1.21.1 N18Q/N106Q enzyme activity is lower than those of the single mutants, is unstable to heat and is the most sensitive to trypsin Homo sapiens
3.1.21.1 N18Q/N106Q enzyme activity is lower than those of the single mutants, is unstable to heat and is the most sensitive to trypsin Equus caballus
3.1.21.1 N18Q/N106Q enzyme activity is lower than those of the single mutants, is unstable to heat, trypsin resistance decreases in a time-dependent manner Bos taurus

General Stability

EC Number General Stability Organism
3.1.21.1 N18 and N106 are both necessary for full enzymatic activity, heat-stability, and trypsin resistance Bos taurus
3.1.21.1 N18 and N106 are both necessary for full enzymatic activity, heat-stability, and trypsin resistance Homo sapiens
3.1.21.1 N18 and N106 are both necessary for full enzymatic activity, heat-stability, and trypsin resistance Equus caballus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 additional information is resistant to trypsin Homo sapiens
3.1.21.1 Trypsin is less resistant to trypsin than human DNase I, DNase I activity decreases gradually Bos taurus
3.1.21.1 Trypsin is less resistant to trypsin than human DNase I, DNase I activity decreases gradually Equus caballus

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Bos taurus
-
-
-
3.1.21.1 Equus caballus Q4AEE3
-
-
3.1.21.1 Homo sapiens P24855
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 additional information pancreas-parotid Bos taurus
-
3.1.21.1 pancreas
-
 Homo sapiens
-
3.1.21.1 parotid gland
-
 Equus caballus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 salmon testis DNA + H2O
-
 Bos taurus 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 salmon testis DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
3.1.21.1 salmon testis DNA + H2O
-
 Equus caballus 5'-phosphooligonucleotides + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.1 deoxyribonuclease I
-
 Bos taurus
3.1.21.1 deoxyribonuclease I
-
 Homo sapiens
3.1.21.1 deoxyribonuclease I
-
 Equus caballus
3.1.21.1 DNase I
-
 Bos taurus
3.1.21.1 DNase I
-
 Homo sapiens
3.1.21.1 DNase I
-
 Equus caballus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.1 additional information
-
 both N-linked glycosylation sites N18 and N106 are required for heat stability Bos taurus
3.1.21.1 additional information
-
 both N-linked glycosylation sites N18 and N106 are required for heat stability Homo sapiens
3.1.21.1 additional information
-
 both N-linked glycosylation sites N18 and N106 are required for heat stability Equus caballus