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Literature summary extracted from

  • Cao, Y.; Huang, H.; Meng, K.; Yang, P.; Shi, P.; Wang, Y.; Luo, H.; Zhang, Z.; Wu, N.; Yao, B.
    Cloning and functional expression of an alpha-galactosidase from Yersinia pestis biovar Microtus str. 91001 (2008), Biosci. Biotechnol. Biochem., 72, 2203-2205.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expression in Escherichia coli Yersinia pestis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ inactivation Yersinia pestis
3.2.1.22 Hg2+ inactivation Yersinia pestis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.00135
-
 melibiose
-
 Yersinia pestis
3.2.1.22 0.51
-
 4-nitrophenyl alpha-D-galactoside
-
 Yersinia pestis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 80000
-
 x * 80000, SDS-PAGE Yersinia pestis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Yersinia pestis
-
 biovar Microtus str. 91001
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 21.2
-
 recombinant enzyme Yersinia pestis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-methylumbelliferyl-alpha-D-galactoside + H2O
-
 Yersinia pestis 4-methylumbelliferol + alpha-D-galactose
-
 ?
3.2.1.22 4-nitrophenyl alpha-D-galactoside + H2O
-
 Yersinia pestis 4-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 melibiose + H2O
-
 Yersinia pestis D-galactose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 ? x * 80000, SDS-PAGE Yersinia pestis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 Aga-Y
-
 Yersinia pestis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 37
-
-
 Yersinia pestis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 50
-
 10 min, 3% residual activity Yersinia pestis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 3279
-
 4-nitrophenyl alpha-D-galactoside
-
 Yersinia pestis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 6.8
-
-
 Yersinia pestis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 7
-
 stable in narrow range around pH 7.0 Yersinia pestis