Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Porcal, W.; Hernandez, P.; Boiani, L.; Boiani, M.; Ferreira, A.; Chidichimo, A.; Cazzulo, J.J.; Olea-Azar, C.; Gonzalez, M.; Cerecetto, H.
    New trypanocidal hybrid compounds from the association of hydrazone moieties and benzofuroxan heterocycle (2008), Bioorg. Med. Chem., 16, 6995-7004.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.12 4-(benzofuroxan-5-ylmethyloxy)benzaldehyde amidinohydrazone 50% of inhibition at a inhibitor dose of 0.1 mM, 30% of inhibition at a inhibitor dose of 0.04 mM Trypanosoma cruzi
1.8.1.12 benzofuroxan-5-carboxaldehyde thiosemicarbazone 19% of inhibition at a inhibitor dose of 0.1 mM, 9% of inhibition at a inhibitor dose of 0.04 mM Trypanosoma cruzi
1.8.1.12 N4-allyl benzofuroxan-5-carboxaldehyde thiosemicarbazone 9% of inhibition at a inhibitor dose of 0.1 mM, 13% of inhibition at a inhibitor dose of 0.04 mM Trypanosoma cruzi
3.4.22.51 2-[(benzofuroxan-5-yl)methylene]-1-(2-hydroxybenzylidene)hydrazine
-
 Trypanosoma cruzi
3.4.22.51 2-[(benzofuroxan-5-yl)methylene]-1-(4-hydroxybenzylidene)hydrazine
-
 Trypanosoma cruzi
3.4.22.51 2-[(benzofuroxan-5-yl)methylene]-1-(5-bromo-2-hydroxybenzylidene)hydrazine
-
 Trypanosoma cruzi
3.4.22.51 4-(benzofuroxan-5-ylmethyloxy)benzaldehyde amidinohydrazone
-
 Trypanosoma cruzi
3.4.22.51 4-(benzofuroxan-5-ylmethyloxy)benzaldehyde semicarbazone 0.1 mM, 36% of inhibition Trypanosoma cruzi
3.4.22.51 4-(benzofuroxan-5-ylmethyloxy)benzaldehyde thiosemicarbazone
-
 Trypanosoma cruzi
3.4.22.51 benzofuroxan-5-carboxaldehyde thiosemicarbazone 0.1 mM, 7% of inhibition Trypanosoma cruzi
3.4.22.51 N-phenyl benzofuroxan-5-carboxaldehyde thiosemicarbazone
-
 Trypanosoma cruzi
3.4.22.51 N4-allyl benzofuroxan-5-carboxaldehyde thiosemicarbazone 0.1 mM, 36% of inhibition Trypanosoma cruzi
3.4.22.51 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
 Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.12 Trypanosoma cruzi
-
-
-
3.4.22.51 Trypanosoma cruzi
-
 Tulahuen 2 strain
-
3.4.22.51 Trypanosoma cruzi Tulahuen 2
-
 Tulahuen 2 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.51
-
 Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.12 NADPH + trypanothione disulfide + H+ 100 microM NADPH, 105 or 93 microM trypanothione disulfide, pH 7.5, 25°C Trypanosoma cruzi trypanothione + NADP+
-
 ?
1.8.1.12 NADPH + trypanothione disulfide + H+ 100 microM NADPH, 105 or 93 microM trypanothione disulfide, pH 7.5, 25°C Trypanosoma cruzi Tulahuen 2 trypanothione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.12 trypanothione reductase
-
 Trypanosoma cruzi
3.4.22.51 CP
-
 Trypanosoma cruzi
3.4.22.51 cruzipain
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.12 25
-
 assay at pH 7.5 Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.12 7.5
-
 assay at 25°C Trypanosoma cruzi
3.4.22.51 8
-
 30 min, 50 mM Tris-acetate buffer, 0.3 mM Bz-Pro-Phe-Arg-pNA, 10 mM beta-mercaptoethanol Trypanosoma cruzi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.22.51 0.032
-
-
 Trypanosoma cruzi 4-(benzofuroxan-5-ylmethyloxy)benzaldehyde amidinohydrazone
3.4.22.51 0.043
-
-
 Trypanosoma cruzi 4-(benzofuroxan-5-ylmethyloxy)benzaldehyde thiosemicarbazone
3.4.22.51 0.068
-
-
 Trypanosoma cruzi 2-[(benzofuroxan-5-yl)methylene]-1-(2-hydroxybenzylidene)hydrazine
3.4.22.51 0.073
-
-
 Trypanosoma cruzi 2-[(benzofuroxan-5-yl)methylene]-1-(5-bromo-2-hydroxybenzylidene)hydrazine
3.4.22.51 0.078
-
-
 Trypanosoma cruzi N-phenyl benzofuroxan-5-carboxaldehyde thiosemicarbazone
3.4.22.51 0.1
-
-
 Trypanosoma cruzi 2-[(benzofuroxan-5-yl)methylene]-1-(4-hydroxybenzylidene)hydrazine
3.4.22.51 0.1
-
 above 0.1 mM Trypanosoma cruzi benzofuroxan-5-carboxaldehyde thiosemicarbazone
3.4.22.51 0.1
-
 above 0.1 mM Trypanosoma cruzi N4-allyl benzofuroxan-5-carboxaldehyde thiosemicarbazone
3.4.22.51 0.1
-
 above 0.1 mM Trypanosoma cruzi 4-(benzofuroxan-5-ylmethyloxy)benzaldehyde semicarbazone