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Literature summary extracted from

  • Magor, A.M.; Venables, W.A.
    Solubilization, purification and characterization of D-alanine dehydrogenase from Pseudomonas aeruginosa and effects of solubilization on its properties (1987), Biochimie, 69, 63-69.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.5.1 association with the membrane bilayer protects the enzyme against thermal denaturation Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.5.1 15
-
 D-alanine purified preparation Pseudomonas aeruginosa
1.4.5.1 26
-
 D-alanine solubilized enzyme Pseudomonas aeruginosa
1.4.5.1 53
-
 D-alanine native enzyme Pseudomonas aeruginosa
1.4.5.1 71
-
 D-glutamine
-
 Pseudomonas aeruginosa
1.4.5.1 200
-
 D-cycloserine
-
 Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.5.1 membrane
-
 Pseudomonas aeruginosa 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.5.1 49000
-
 SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.4.5.1 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.5.1
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.5.1 0.653
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.5.1 D-2-amino-n-butyrate + oxidized 2,6-dichlorophenolindophenol + H2O 81% of the activity with D-alanine Pseudomonas aeruginosa 2-oxobutyrate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
1.4.5.1 D-alanine + oxidized 2,6-dichloroindophenol + H2O
-
 Pseudomonas aeruginosa pyruvate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
1.4.5.1 D-arginine + oxidized 2,6-dichlorophenolindophenol + H2O 41% of the activity with D-alanine Pseudomonas aeruginosa 2-oxo-5-guanidinopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.5.1 D-asparagine + oxidized 2,6-dichlorophenolindophenol + H2O 96% of the activity with D-alanine Pseudomonas aeruginosa 2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.5.1 D-cycloserine + oxidized 2,6-dichloroindophenol + H2O as a active as D-alanine Pseudomonas aeruginosa ?
-
 ?
1.4.5.1 D-glutamine + oxidized 2,6-dichloroindophenol + H2O 52% of the activity with D-alanine Pseudomonas aeruginosa 4-carbamoyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
1.4.5.1 D-methionine + oxidized 2,6-dichlorophenolindophenol + H2O 70% of the activity with D-alanine Pseudomonas aeruginosa 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.5.1 D-phenylalanine + oxidized 2,6-dichloroindophenol + H2O 63% of the activity with D-alanine Pseudomonas aeruginosa phenylpyruvate + NH3 + reduced 2,6-dichloroindophenol
-
 ?
1.4.5.1 D-proline + oxidized 2,6-dichlorophenolindophenol 19% of the activity with D-alanine Pseudomonas aeruginosa DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.5.1 D-valine + oxidized 2,6-dichlorophenolindophenol + H2O 26% of the activity with D-alanine Pseudomonas aeruginosa 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.5.1 monomer 1 * 49000, SDS-PAGE Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.5.1 additional information
-
 association with the membrane bilayer protects the enzyme against thermal denaturation Pseudomonas aeruginosa
1.4.5.1 37
-
 60 min, solubilized enzyme loses 98% of its initial activity, the native enzyme loses 47% of its activity Pseudomonas aeruginosa
1.4.5.1 42
-
 10 min, solubilized enzyme loses 95% of its activity, the native enzyme loses 49% of its activity Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.5.1 8.5
-
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.5.1 FAD flavoprotein Pseudomonas aeruginosa