EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.13.9 | expressed in Escherichia coli Top10F' cells | Lactococcus lactis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.13.9 | H303S/S307G | inactive | Lactococcus lactis |
3.4.13.9 | R293S | the mutation results in the disappearance of the allosteric behaviour yielding a Hill constant of 0.98 while the wild type has a constant of 1.58 and suppresses the substrate inhibition that is observed in other mutants and wild type enzyme, the Km value for L-Leu-L-Pro is 2.9fold larger and Vmax is approximately 50% less as compared to the wild type enzyme | Lactococcus lactis |
3.4.13.9 | R293S/S307G | mutant shows strongly reduced specific activity towards L-Leu-L-Pro compared to the wild type enzyme | Lactococcus lactis |
3.4.13.9 | S307D | mutant shows reduced specific activity towards L-Leu-L-Pro compared to the wild type enzyme | Lactococcus lactis |
3.4.13.9 | S307G | mutant shows reduced specific activity towards L-Leu-L-Pro compared to the wild type enzyme | Lactococcus lactis |
3.4.13.9 | S307R | mutant shows reduced specific activity towards L-Leu-L-Pro compared to the wild type enzyme | Lactococcus lactis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.9 | L-Leu-L-Pro | - |
Lactococcus lactis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.13.9 | 1.7 | - |
L-Leu-L-Pro | mutant enzyme S307G, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C | Lactococcus lactis | |
3.4.13.9 | 8 | - |
L-Leu-L-Pro | wild type enzyme, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C | Lactococcus lactis | |
3.4.13.9 | 10.1 | - |
L-Leu-L-Pro | mutant enzyme R293S/S307G, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C | Lactococcus lactis | |
3.4.13.9 | 14.7 | - |
L-Leu-L-Pro | mutant enzyme S307R, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C | Lactococcus lactis | |
3.4.13.9 | 18 | - |
L-Leu-L-Pro | mutant enzyme S307D, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C | Lactococcus lactis | |
3.4.13.9 | 23.3 | - |
L-Leu-L-Pro | mutant enzyme R293S, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C | Lactococcus lactis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.9 | Zn2+ | required for optimum activity | Lactococcus lactis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.13.9 | Lactococcus lactis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.13.9 | DEAE Sephacel column chromatography | Lactococcus lactis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.13.9 | 0.33 | - |
mutant enzyme S307R, in the presence of 1 mM ZnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 0.48 | - |
mutant enzyme S307D, in the presence of 1 mM ZnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 0.64 | - |
mutant enzyme R293S, in the presence of 1 mM MnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 1.1 | - |
mutant enzyme S307D, in the presence of 1 mM ZnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 1.4 | - |
mutant enzyme R293S, in the presence of 1 mM ZnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 1.4 | - |
mutant enzyme S307D, in the presence of 1 mM MnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 1.7 | - |
mutant enzyme S307D, in the presence of 1 mM ZnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 2 | - |
mutant enzyme R293S, in the presence of 1 mM MnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 2.7 | - |
mutant enzyme S307D, in the presence of 1 mM ZnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 3 | 3.9 | mutant enzyme R293S/S307G, in the presence of 1 mM MnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 3.2 | - |
mutant enzyme R293S, in the presence of 1 mM ZnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 3.2 | - |
mutant enzyme S307D, in the presence of 1 mM MnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 4.3 | - |
mutant enzyme R293S, in the presence of 1 mM ZnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 4.4 | - |
mutant enzyme S307G, in the presence of 1 mM ZnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 4.8 | - |
mutant enzyme R293S, in the presence of 1 mM ZnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 5 | - |
mutant enzyme R293S, in the presence of 1 mM MnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 5.2 | - |
mutant enzyme S307D, in the presence of 1 mM MnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 5.3 | - |
mutant enzyme R293S, in the presence of 1 mM MnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 5.9 | - |
mutant enzyme R293S, in the presence of 1 mM MnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 6.2 | - |
mutant enzyme S307R, in the presence of 1 mM MnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 7 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM ZnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 7.8 | - |
mutant enzyme S307G, in the presence of 1 mM ZnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 8.2 | - |
mutant enzyme S307R, in the presence of 1 mM ZnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 8.9 | - |
mutant enzyme S307R, in the presence of 1 mM MnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 9.6 | - |
mutant enzyme S307G, in the presence of 1 mM MnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 9.6 | - |
mutant enzyme S307G, in the presence of 1 mM MnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 10.6 | - |
mutant enzyme S307R, in the presence of 1 mM ZnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 11.6 | - |
mutant enzyme S307D, in the presence of 1 mM MnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 11.9 | - |
mutant enzyme S307R, in the presence of 1 mM ZnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 12.4 | - |
mutant enzyme S307G, in the presence of 1 mM ZnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 12.8 | - |
mutant enzyme S307G, in the presence of 1 mM ZnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 13.4 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM MnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 13.6 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM ZnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 18.4 | - |
mutant enzyme S307R, in the presence of 1 mM MnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 22.7 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM MnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 25.6 | - |
mutant enzyme S307D, in the presence of 1 mM MnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 27.3 | - |
wild type enzyme, in the presence of 1 mM MnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 27.6 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM MnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 30.9 | - |
wild type enzyme, in the presence of 1 mM ZnCl2, using L-Lys-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 31.5 | - |
mutant enzyme S307D, in the presence of 1 mM ZnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 32.3 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM ZnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 32.7 | - |
mutant enzyme S307G, in the presence of 1 mM MnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 36.9 | - |
mutant enzyme S307G, in the presence of 1 mM MnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 42.3 | - |
mutant enzyme S307R, in the presence of 1 mM MnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 46.7 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM ZnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 48.3 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM MnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 53.7 | - |
mutant enzyme S307G, in the presence of 1 mM MnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 56.2 | - |
wild type enzyme, in the presence of 1 mM ZnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 64.9 | - |
mutant enzyme R293S, in the presence of 1 mM ZnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 67.7 | - |
wild type enzyme, in the presence of 1 mM ZnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 78.2 | - |
mutant enzyme R293S/S307G, in the presence of 1 mM ZnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 81.8 | - |
mutant enzyme S307G, in the presence of 1 mM ZnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 87.8 | - |
mutant enzyme S307R, in the presence of 1 mM ZnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 100.8 | - |
mutant enzyme S307R, in the presence of 1 mM MnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 111.4 | - |
wild type enzyme, in the presence of 1 mM ZnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 159.7 | - |
wild type enzyme, in the presence of 1 mM MnCl2, using L-Val-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 166.1 | - |
wild type enzyme, in the presence of 1 mM MnCl2, using L-Phe-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 234.5 | - |
wild type enzyme, in the presence of 1 mM MnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 463.7 | - |
wild type enzyme, in the presence of 1 mM MnCl2, using L-Arg-L-Pro as substrate, at 50°C | Lactococcus lactis |
3.4.13.9 | 468.7 | - |
wild type enzyme, in the presence of 1 mM ZnCl2, using L-Leu-L-Pro as substrate, at 50°C | Lactococcus lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.13.9 | L-Arg-L-Pro + H2O | 12.0% activity compared to L-Leu-L-Pro, in the presence of 1 mM ZnCl2 | Lactococcus lactis | L-Arg + L-Pro | - |
? | |
3.4.13.9 | L-Leu-L-Pro + H2O | 100% activity in the presence of 1 mM ZnCl2 | Lactococcus lactis | L-Leu + L-Pro | - |
? | |
3.4.13.9 | L-Lys-L-Pro + H2O | 6.6% activity compared to L-Leu-L-Pro, in the presence of 1 mM ZnCl2 | Lactococcus lactis | L-Lys + L-Pro | - |
? | |
3.4.13.9 | L-Phe-L-Pro + H2O | 23.8% activity compared to L-Leu-L-Pro, in the presence of 1 mM ZnCl2 | Lactococcus lactis | L-Phe + L-Pro | - |
? | |
3.4.13.9 | L-Val-L-Pro + H2O | 14.4% activity compared to L-Leu-L-Pro, in the presence of 1 mM ZnCl2 | Lactococcus lactis | L-Val + L-Pro | - |
? | |
3.4.13.9 | additional information | does not degrade L-Glu-L-Pro, Gly-L-Pro, L-Pro-L-Pro, L-Leu-L-Leu-L-Pro, L-Leu-L-Val-L-Pro, and L-Asp-L-Pro | Lactococcus lactis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.13.9 | prolidase | - |
Lactococcus lactis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.13.9 | 15.3 | - |
L-Leu-L-Pro | wild type enzyme, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C | Lactococcus lactis |