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Literature summary extracted from

  • Crobu, D.; Canevari, G.; Milani, M.; Pandini, V.; Vanoni, M.A.; Bolognesi, M.; Zanetti, G.; Aliverti, A.
    Plasmodium falciparum ferredoxin-NADP+ reductase His286 plays a dual role in NADP(H) binding and catalysis (2009), Biochemistry, 48, 9525-9533.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 overexpressed in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.1.2
-
 Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.2 H286A kcat and kcat/Km are markedly reduced Plasmodium falciparum
1.18.1.2 H286K considerable decrease in kcat and kcat/Km, almost no reactivity with K3Fe(CN)6 Plasmodium falciparum
1.18.1.2 H286L kcat and kcat/Km are markedly reduced, anomalous reactivity with 2,6-dichlorophenolindophenol Plasmodium falciparum
1.18.1.2 H286Q up to 50% higher kcat than wild type, higher kcat/Km when 2,6-dichlorophenolindophenol serves as electron acceptor Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.2 2'-AMP when 2,6-dichlorophenolindophenol serves as electron acceptor Plasmodium falciparum
1.18.1.2 K3Fe(CN)6
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.022
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q Plasmodium falciparum
1.18.1.2 0.036
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor Plasmodium falciparum
1.18.1.2 0.057
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q Plasmodium falciparum
1.18.1.2 0.071
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor Plasmodium falciparum
1.18.1.2 0.14
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K Plasmodium falciparum
1.18.1.2 0.37
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L Plasmodium falciparum
1.18.1.2 0.4
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A Plasmodium falciparum
1.18.1.2 0.52
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Plasmodium falciparum C6KT68
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Plasmodium falciparum NADP+ + reduced 2,6-dichlorophenolindophenol
-
 r
1.18.1.2 NADPH + K3Fe(CN)6 assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Plasmodium falciparum NADP+ + reduced K3Fe(CN)6
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ reductase
-
 Plasmodium falciparum
1.18.1.2 FNR
-
 Plasmodium falciparum
1.18.1.2 PfFNR ferredoxin-NADP+ reductase of Plasmodium falciparum Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 3.8
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286K Plasmodium falciparum
1.18.1.2 6
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K Plasmodium falciparum
1.18.1.2 7.5
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L Plasmodium falciparum
1.18.1.2 17.5
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A Plasmodium falciparum
1.18.1.2 44.5
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A Plasmodium falciparum
1.18.1.2 55
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor Plasmodium falciparum
1.18.1.2 70
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q Plasmodium falciparum
1.18.1.2 125
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor Plasmodium falciparum
1.18.1.2 185
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.18.1.2 0.024
-
 2'-AMP when 2,6-dichlorophenolindophenol serves as electron acceptor Plasmodium falciparum
1.18.1.2 0.23
-
 K3Fe(CN)6
-
 Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 20
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L Plasmodium falciparum
1.18.1.2 45
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K Plasmodium falciparum
1.18.1.2 49
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A Plasmodium falciparum
1.18.1.2 85
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A Plasmodium falciparum
1.18.1.2 750
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor Plasmodium falciparum
1.18.1.2 3150
-
 NADPH in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q Plasmodium falciparum
1.18.1.2 3300
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q Plasmodium falciparum
1.18.1.2 3500
-
 NADPH in an assay using K3Fe(CN)6 as electron acceptor Plasmodium falciparum