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Literature summary extracted from

  • Cervellati, C.; Li, L.; Andi, B.; Guariento, A.; Dallocchio, F.; Cook, P.F.
    Proper orientation of the nicotinamide ring of NADP is important for the precatalytic conformational change in the 6-phosphogluconate dehydrogenase reaction (2008), Biochemistry, 47, 1862-1870.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.44 expressed in Escherichia coli M15 cells Ovis aries

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.44 E131A the mutant shows a 2.3fold decrease in turnover number compared to the wild type enzyme Ovis aries
1.1.1.44 M13C the mutant shows a decrease in turnover number compared to the wild type enzyme Ovis aries
1.1.1.44 M13Q the mutant shows a 8.1fold decrease in turnover number compared to the wild type enzyme Ovis aries
1.1.1.44 M13V the mutant shows a decrease in turnover number compared to the wild type enzyme Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44 Ni-NTA column chromatography and ADP-agarose column chromatography Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 liver
-
 Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 2-deoxy-6-phospho-D-gluconate + NADP+
-
 Ovis aries 3-oxo-2-deoxy-6-phosphogluconate + NADPH
-
 ?
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Ovis aries
1.1.1.44 6PGDH
-
 Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+
-
 Ovis aries