EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.75 | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.75 | 0.000096 | - |
adenosine 37 in tRNA-Phe | pH 7.5, 37°C | Escherichia coli | |
2.5.1.75 | 0.0032 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.75 | 34700 | - |
1 * 34700, SDS-PAGE | Escherichia coli |
2.5.1.75 | 37800 | - |
gel fitlration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.75 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.75 | recombinant enzyme | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.75 | dimethylallyl diphosphate + adenine37 in tRNA = diphosphate + N6-dimethylallyladenine37 in tRNA | ordered sequential mechanism for substrate binding | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.75 | 1.15 | - |
pH 7.5, 37°C | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.75 | -78°C, stable for several months | Escherichia coli |
2.5.1.75 | 0°C, stable for at least one week | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.75 | dimethylallyl diphosphate + adenine37 in tRNAPhe | - |
Escherichia coli | diphosphate + N6-dimethylallyladenine37 in tRNAPhe | enzyme transfers the dimethylallyl moiety of dimethylallyl diphosphate to A37, located adjacent to the anticodon in undermodified tRNAPhe | ? | |
2.5.1.75 | dimethylallyl diphosphate + adenosine 37 in tRNA-Phe | - |
Escherichia coli | N6-(dimethylallyl)adenosine37 in tRNAPhe + diphosphate | - |
? | |
2.5.1.75 | additional information | enzyme does not bind dimethylallyl diphosphate in absence of tRNA | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.75 | monomer | 1 * 34700, SDS-PAGE | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.75 | 6.5 | 9 | broad | Escherichia coli |