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Literature summary extracted from

  • Tikhonova, T.V.; Slutskaya, E.S.; Filimonenkov, A.A.; Boyko, K.M.; Kleimenov, S.Y.; Konarev, P.V.; Polyakov, K.M.; Svergun, D.I.; Trofimov, A.A.; Khomenkov, V.G.; Zvyagilskaya, R.A.; Popov, V.O.
    Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens (2008), Biochemistry (Moscow), 73, 164-170.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.2 soluble
-
 Thioalkalivibrio nitratireducens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.2 Iron [4Fe-4S]-ferredoxin cofactor Thioalkalivibrio nitratireducens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.2 380000 390000 gel filtration Thioalkalivibrio nitratireducens

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.2 Thioalkalivibrio nitratireducens Q5F2I3 a haloalkaliphilic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.2 native enzyme 57.7fold by anion exchange chromatography and gel filtration Thioalkalivibrio nitratireducens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.2 150
-
 purified native enzyme Thioalkalivibrio nitratireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.2 nitrite + reduced methyl viologen
-
 Thioalkalivibrio nitratireducens NH3 + H2O + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.2 hexamer determination of the oligomeric composition of NiR by small-angle X-ray scattering, overview Thioalkalivibrio nitratireducens

Synonyms

EC Number Synonyms Comment Organism
1.7.2.2 cytochrome c nitrite reductase
-
 Thioalkalivibrio nitratireducens
1.7.2.2 NiR
-
 Thioalkalivibrio nitratireducens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.2 30
-
 assay at Thioalkalivibrio nitratireducens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.2.2 50 70 at least 50 h, purified enzyme, stable Thioalkalivibrio nitratireducens
1.7.2.2 80
-
 the half-life is 2.2 h, enzyme inactivation is described by a two-step scheme, about 20% residual activity of after 25 h Thioalkalivibrio nitratireducens
1.7.2.2 86
-
 melting of the TvNiR molecule is an irreversible highly cooperative process with the transition temperature of 86°C and the transition enthalpy of about J/mol of hexamer Thioalkalivibrio nitratireducens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.2 7
-
 assay at Thioalkalivibrio nitratireducens

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.2 Ferredoxin [4Fe-4S]-ferredoxin cofactor Thioalkalivibrio nitratireducens
1.7.2.2 methyl viologen used as the electron donor, reduced with dithionite, artificial cofactor Thioalkalivibrio nitratireducens