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Literature summary extracted from

  • Sivuk, V.F.; Rusina, I.M.; Makarchikov, A.F.
    Purification and characteristics of functional properties of soluble nucleoside triphosphatase (apyrase) from bovine brain (2008), Biochemistry (Moscow), 73, 1047-1052.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.5 2-oxoglutarate activates to 148% at 0.5 mM Bos taurus
3.6.1.5 acetyl-CoA activates to 148% at 0.5 mM Bos taurus
3.6.1.5 D-fructose-1,6-diphosphate activates to 155% at 0.5 mM Bos taurus
3.6.1.15 2-oxoglutarate stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 128% relative activity at a concentration of 0.05 mM Bos taurus
3.6.1.15 acetyl-CoA stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 127% relative activity at a concentration of 0.05 mM Bos taurus
3.6.1.15 D-fructose 1,6-bisphosphate stimulating in physiological concentrations, 155% relative activity at a concentration of 0.5 mM, 126% relative activity at a concentration of 0.05 mM Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 ADP 75% inhibition at 0.5 mM Bos taurus
3.6.1.5 AMP complete inhibition at 0.5 mM Bos taurus
3.6.1.5 ATP 68% inhibition at 0.5 mM Bos taurus
3.6.1.5 CDP 59% inhibition at 0.5 mM Bos taurus
3.6.1.5 citrate 20% inhibition at 0.5 mM Bos taurus
3.6.1.5 CMP complete inhibition at 0.5 mM Bos taurus
3.6.1.5 GDP 81% inhibition at 0.5 mM Bos taurus
3.6.1.5 GMP complete inhibition at 0.5 mM Bos taurus
3.6.1.5 GTP 70% inhibition at 0.5 mM Bos taurus
3.6.1.5 IDP 59% inhibition at 0.5 mM Bos taurus
3.6.1.5 IMP 95% inhibition at 0.5 mM Bos taurus
3.6.1.5 ITP 42% inhibition at 0.5 mM Bos taurus
3.6.1.5 propionate 61% inhibition at 0.5 mM Bos taurus
3.6.1.5 succinate 55% inhibition at 0.5 mM Bos taurus
3.6.1.5 UDP 68% inhibition at 0.5 mM Bos taurus
3.6.1.5 UMP 92% inhibition at 0.5 mM Bos taurus
3.6.1.5 UTP inhibitory at 0.6 mM and above, 34% inhibition at 0.5 mM Bos taurus
3.6.1.5 XTP 60% inhibition at 0.5 mM Bos taurus
3.6.1.15 propionate 39% relative activity at a concentration of 0.5 mM, 63% relative activity at a concentration of 0.05 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 additional information
-
 additional information the enzyme shows Michaelis-Menten kinetics with all substrates except for UTP Bos taurus
3.6.1.15 0.025
-
 ITP in presence of 10 mM Mg2+ Bos taurus
3.6.1.15 0.041
-
 GTP in presence of 10 mM Mg2+ Bos taurus
3.6.1.15 0.086
-
 UTP in presence of 10 mM Mg2+ Bos taurus
3.6.1.15 0.15
-
 CTP in presence of 10 mM Mg2+ Bos taurus
3.6.1.15 0.26
-
 ATP in presence of 10 mM Mg2+ Bos taurus
3.6.1.15 0.5
-
 CDP in presence of 10 mM Mg2+ Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.5 cytosol
-
 Bos taurus 5829
-
3.6.1.15 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ activates Bos taurus
3.6.1.5 Co2+ activates Bos taurus
3.6.1.5 Cu2+ activates Bos taurus
3.6.1.5 Mg2+ activates, best metal ion Bos taurus
3.6.1.5 Mn2+ activates Bos taurus
3.6.1.5 additional information enzyme activity is absolutely dependent on divalent metal ions, Mg2+ exhibits the maximal activating effect among the studied cations. Other metal cations like Mn2+, Co2+, Ca2+, Cu2+, and Zn2+ are less efficient, and Ba2+ is not able to activate the enzyme Bos taurus
3.6.1.5 Zn2+ activates Bos taurus
3.6.1.15 Ba2+ 5 mM, unable to stimulate enzyme activity Bos taurus
3.6.1.15 Ca2+ 5 mM, 52% relative enzyme activity in presence of Bos taurus
3.6.1.15 Co2+ 5 mM, 60% relative enzyme activity in presence of Bos taurus
3.6.1.15 Cu2+ 5 mM, 46% relative enzyme activity in presence of Bos taurus
3.6.1.15 Mg2+ 5 mM, 100% relative enzyme activity in presence of Bos taurus
3.6.1.15 Mn2+ 5 mM, 73% relative enzyme activity in presence of Bos taurus
3.6.1.15 Zn2+ 5 mM, 34% relative enzyme activity in presence of Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 58600
-
 gel filtration Bos taurus
3.6.1.5 60200
-
 1 * 60200, SDS-PAGE Bos taurus
3.6.1.15 58600
-
 gel filtration, at 4°C in 20 mM Tris-HCl, 0.1 M NaCl, pH 7.5 Bos taurus
3.6.1.15 60200
-
 SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.15 ADP + H2O Bos taurus 0.5 mM, 25% relative activity towards ADP compared to CTP, no activity towards AMP AMP + phosphate
-
 ?
3.6.1.15 ATP + H2O Bos taurus 0.5 mM, 32% relative activity compared to CTP ADP + phosphate
-
 ?
3.6.1.15 CDP + H2O Bos taurus 0.5 mM, 41% relative activity towards CDP, no activity towards CMP CMP + phosphate
-
 ?
3.6.1.15 CTP + H2O Bos taurus 0.5 mM, 100% relative activity CDP + phosphate
-
 ?
3.6.1.15 GDP + H2O Bos taurus 0.5 mM, 19% relative activity towards GDP compared to CTP, no activity towards GMP GMP + phosphate
-
 ?
3.6.1.15 GTP + H2O Bos taurus 0.5 mM, 30% relative activity compared to CTP GDP + phosphate
-
 ?
3.6.1.15 IDP + H2O Bos taurus 0.5 mM, 41% relative activity towards IDP compared to CTP, 5.3% relative activity towards IMP compared to CTP IMP + phosphate
-
 ?
3.6.1.15 ITP + H2O Bos taurus 0.5 mM, 58% relative activity compared to CTP IDP + phosphate
-
 ?
3.6.1.15 UDP + H2O Bos taurus 0.5 mM, 32% relative activity compared to CTP, 8.2% relative activity towards UMP compared to CTP UMP + phosphate
-
 ?
3.6.1.15 UTP + H2O Bos taurus 0.5 mM, 66% relative activity compared to CTP UDP + phosphate
-
 ?
3.6.1.15 XTP + H2O Bos taurus 0.5 mM, 40% relative activity compared to CTP XDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Bos taurus
-
-
-
3.6.1.15 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5 native enzyme 4915fold from brain by ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, anion exchange and hydroxyapatite chromatography, again gel filtration, and adsorption chromatography Bos taurus
3.6.1.15 gel filtration, SDS-PAGE Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 brain
-
 Bos taurus
-
3.6.1.5 erythrocyte
-
 Bos taurus
-
3.6.1.5 heart
-
 Bos taurus
-
3.6.1.5 kidney low enzyme activity Bos taurus
-
3.6.1.5 liver high enzyme activity Bos taurus
-
3.6.1.5 lung
-
 Bos taurus
-
3.6.1.5 skeletal muscle
-
 Bos taurus
-
3.6.1.5 small intestine low enzyme activity Bos taurus
-
3.6.1.5 spleen
-
 Bos taurus
-
3.6.1.15 brain purified from Bos taurus
-
3.6.1.15 heart expression in Bos taurus
-
3.6.1.15 kidney expression in Bos taurus
-
3.6.1.15 liver expression in Bos taurus
-
3.6.1.15 lung expression in Bos taurus
-
3.6.1.15 small intestine expression in Bos taurus
-
3.6.1.15 spleen expression in Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 additional information
-
 tissue-specific activity, overview Bos taurus
3.6.1.5 0.09
-
 erythrocytes Bos taurus
3.6.1.5 0.47
-
 liver Bos taurus
3.6.1.5 639
-
 purifed native enzyme from brain Bos taurus
3.6.1.15 additional information
-
 dependence on bivalent cations and broad substrate specificity towards nucleoside-5'-tri- and -diphosphates reveal similarities to apyrases Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ATP + 2 H2O
-
 Bos taurus AMP + 2 phosphate
-
 ?
3.6.1.5 CDP + H2O
-
 Bos taurus CMP + phosphate
-
 ?
3.6.1.5 CTP + 2 H2O
-
 Bos taurus CMP + 2 phosphate
-
 ?
3.6.1.5 GTP + 2 H2O
-
 Bos taurus GMP + 2 phosphate
-
 ?
3.6.1.5 ITP + 2 H2O
-
 Bos taurus IMP + 2 phosphate
-
 ?
3.6.1.5 UTP + 2 H2O
-
 Bos taurus UMP + 2 phosphate
-
 ?
3.6.1.15 ADP + H2O 0.5 mM, 25% relative activity towards ADP compared to CTP, no activity towards AMP Bos taurus AMP + phosphate
-
 ?
3.6.1.15 ATP + H2O 0.5 mM, 32% relative activity compared to CTP Bos taurus ADP + phosphate
-
 ?
3.6.1.15 CDP + H2O 0.5 mM, 41% relative activity towards CDP, no activity towards CMP Bos taurus CMP + phosphate
-
 ?
3.6.1.15 CDP + H2O 0.5 mM, 41% relative activity towards CDP compared to CTP, no activity towards CMP Bos taurus CMP + phosphate
-
 ?
3.6.1.15 CTP + H2O 0.5 mM, 100% relative activity Bos taurus CDP + phosphate
-
 ?
3.6.1.15 GDP + H2O 0.5 mM, 19% relative activity towards GDP compared to CTP, no activity towards GMP Bos taurus GMP + phosphate
-
 ?
3.6.1.15 GTP + H2O 0.5 mM, 30% relative activity compared to CTP Bos taurus GDP + phosphate
-
 ?
3.6.1.15 IDP + H2O 0.5 mM, 41% relative activity towards IDP compared to CTP, 5.3% relative activity towards IMP compared to CTP Bos taurus IMP + phosphate
-
 ?
3.6.1.15 IDP + H2O 0.5 mM, 41% relative activity towards IDP, 5.3% relative activity towards IMP compared to CTP Bos taurus IMP + phosphate
-
 ?
3.6.1.15 ITP + H2O 0.5 mM, 58% relative activity compared to CTP Bos taurus IDP + phosphate
-
 ?
3.6.1.15 UDP + H2O 0.5 mM, 32% relative activity compared to CTP, 8.2% relative activity towards UMP compared to CTP Bos taurus UMP + phosphate
-
 ?
3.6.1.15 UDP + H2O 0.5 mM, 32% relative activity, 8.2% relative activity towards UMP compared to CTP Bos taurus UMP + phosphate
-
 ?
3.6.1.15 UTP + H2O 0.5 mM, 66% relative activity compared to CTP Bos taurus UDP + phosphate
-
 ?
3.6.1.15 XTP + H2O 0.5 mM, 40% relative activity compared to CTP Bos taurus XDP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.5 monomer 1 * 60200, SDS-PAGE Bos taurus
3.6.1.15 ? x * 60200, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.5 NTPase
-
 Bos taurus
3.6.1.5 nucleoside triphosphatase
-
 Bos taurus
3.6.1.15 nucleoside triphosphatase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.5 37
-
 assay at Bos taurus
3.6.1.15 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 7.5
-
-
 Bos taurus
3.6.1.15 7.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.5 4 10.5
-
 Bos taurus
3.6.1.15 6 8.5 pH 6.0: about 55% of maximal activity, pH 8.5: about 55% of maximal activity Bos taurus