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Literature summary extracted from

  • Graham, D.E.; Taylor, S.M.; Wolf, R.Z.; Namboori, S.C.
    Convergent evolution of coenzyme M biosynthesis in the Methanosarcinales: cysteate synthase evolved from an ancestral threonine synthase (2009), Biochem. J., 424, 467-478.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.76 expression in Escherichia coli Methanosarcina acetivorans
4.1.1.79 expressed in Escherichia coli BL21(DE3) RIL cells containing vector pDG121 Methanosarcina acetivorans
4.2.3.1 His-tagged version expressed in Escherichia coli BL21(DE3) Methanosarcina acetivorans C2A

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.76 additional information expression of truncated protein lacking the first 43 codons of the gene results in an insoluble protein. The refolded protein catalyzes the elimination of phosphate from L-phosphoserine with a rate of 3% of the full-length protein. It forms a monomer of 42300 Da instead of the trimer of the native protein Methanosarcina acetivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.76 DL-2-amino-3-phosphonopropionate
-
 Methanosarcina acetivorans
2.5.1.76 DL-2-Amino-4-phosphonobutanoate
-
 Methanosarcina acetivorans
2.5.1.76 DL-2-amino-5-phosphonopentanoate 1 mM, more than 80% reemaining activity Methanosarcina acetivorans
2.5.1.76 L-aspartate
-
 Methanosarcina acetivorans
2.5.1.76 L-cysteate
-
 Methanosarcina acetivorans
2.5.1.76 L-lysine 1 mM, more than 80% reemaining activity Methanosarcina acetivorans
2.5.1.76 L-serine
-
 Methanosarcina acetivorans
2.5.1.76 L-threonine 1 mM, more than 80% reemaining activity Methanosarcina acetivorans
2.5.1.76 additional information not inhibitory: L-glutamate, D-glutamate, L-glutamine or L-homoserine Methanosarcina acetivorans
2.5.1.76 O-acetyl-L-serine 1 mM, more than 80% reemaining activity Methanosarcina acetivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.76 0.43
-
 L-phosphoserine pH 7.0, 37°C Methanosarcina acetivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.76 46000
-
 3 * 46000, SDS-PAGE Methanosarcina acetivorans
2.5.1.76 132000
-
 gel filtration Methanosarcina acetivorans
4.1.1.79 42300
-
 calculated from amino acid sequence Methanosarcina acetivorans
4.1.1.79 43000
-
 refolded MA3298 protein, SDS-PAGE Methanosarcina acetivorans
4.1.1.79 103000
-
 NusA-tagged MA3298 protein, SDS-PAGE Methanosarcina acetivorans
4.2.3.1 48000
-
 3 * 48000 (calculated 49100), SDS-PAGE, gelfiltration Methanosarcina acetivorans C2A
4.2.3.1 135000
-
 gel filtration Methanosarcina acetivorans C2A

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.76 Methanosarcina acetivorans Q8TKU7
-
-
4.1.1.79 Methanosarcina acetivorans
-
-
-
4.2.3.1 Methanosarcina acetivorans C2A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.79 Ni2+ affinity column chromatography, gel filtration Methanosarcina acetivorans
4.2.3.1 Ni2+-affinity chromatography Methanosarcina acetivorans C2A

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.5.1.76 expression of truncated protein lacking the first 43 codons of the gene results in an insoluble protein. The refolded protein catalyzes the elimination of phosphate from L-phosphoserine with a rate of 3% of the full-length protein Methanosarcina acetivorans
4.1.1.79
-
 Methanosarcina acetivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.76 0.015
-
 pH 7.0, 37°C Methanosarcina acetivorans
4.2.3.1 1.6
-
-
 Methanosarcina acetivorans C2A

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.76 L-phosphoserine + sulfite
-
 Methanosarcina acetivorans L-cysteate + phosphate
-
 ?
2.5.1.76 additional information no reaction in absence of sulfite, no cysteine synthase activity. No substrates are: thiosulfate, nitrite, cyanide or thiocyanide anions Methanosarcina acetivorans ?
-
 ?
4.1.1.79 3-sulfopyruvate
-
 Methanosarcina acetivorans 2-sulfoacetaldehyde + CO2
-
 ?
4.2.3.1 O-phospho-L-homoserine + H2O
-
 Methanosarcina acetivorans C2A L-threonine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.76 trimer 3 * 46000, SDS-PAGE Methanosarcina acetivorans
4.2.3.1 homotrimer 3 * 48000 (calculated 49100), SDS-PAGE, gelfiltration Methanosarcina acetivorans C2A

Synonyms

EC Number Synonyms Comment Organism
2.5.1.76 MA3297
-
 Methanosarcina acetivorans
4.1.1.79 ComDE
-
 Methanosarcina acetivorans
4.1.1.79 MA3298 protein
-
 Methanosarcina acetivorans
4.1.1.79 SpyrDC
-
 Methanosarcina acetivorans
4.2.3.1 threonine synthase (gene MA1610)
-
 Methanosarcina acetivorans C2A

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.76 0.18
-
 L-phosphoserine pH 7.0, 37°C Methanosarcina acetivorans
4.2.3.1 4080
-
 O-phospho-L-homoserine
-
 Methanosarcina acetivorans C2A

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.79 thiamine diphosphate
-
 Methanosarcina acetivorans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.76 1.14
-
 pH 7.0, 37°C Methanosarcina acetivorans DL-2-amino-3-phosphonopropionate
2.5.1.76 12.6
-
 pH 7.0, 37°C Methanosarcina acetivorans L-cysteate
2.5.1.76 15.9
-
 pH 7.0, 37°C Methanosarcina acetivorans L-aspartate
2.5.1.76 16.5
-
 pH 7.0, 37°C Methanosarcina acetivorans DL-2-Amino-4-phosphonobutanoate
2.5.1.76 37.1
-
 pH 7.0, 37°C Methanosarcina acetivorans L-serine