EC Number | Cloned (Comment) | Organism |
---|---|---|
7.5.2.6 | expression of His6-tagged wild-type and mutant enzyme in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.5.2.6 | C315S | site-idrected mutagenesis, structure analysis in comparison to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
7.5.2.6 | C88S | site-directed mutagenesis, structure analysis in comparison to the wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.5.2.6 | additional information | - |
additional information | ligand binding kinetics | Salmonella enterica subsp. enterica serovar Typhimurium | |
7.5.2.6 | 0.41 | - |
ATP | pH 7.5, 37°C, unlabeled recombinant MsbA | Salmonella enterica subsp. enterica serovar Typhimurium | |
7.5.2.6 | 0.52 | - |
ATP | pH 7.5, 37°C, MIANS-labeled recombinant MsbA | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.5.2.6 | membrane | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
7.5.2.6 | 65000 | - |
x * 65000, about, purified recombinant enzyme, mass spectrometry | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.5.2.6 | ATP + H2O + Lipid A/in | Salmonella enterica subsp. enterica serovar Typhimurium | MsbA contains two substrate-binding sites that communicate with both the nucleotide-binding domain and with each other, one is a high affinity binding site for the physiological substrate, lipid A, and the other site interacts with drugs with comparable affinity, overview | ADP + phosphate + Lipid A/out | - |
? | |
7.5.2.6 | ATP + H2O + lipopolysaccharide/in | Salmonella enterica subsp. enterica serovar Typhimurium | MsbA is an essential ABC transporter involved in lipid A transport across the cytoplasmic membrane | ADP + phosphate + lipopolysaccharide/out | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.5.2.6 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene msbA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.5.2.6 | recombinant His6-tagged wild-type and mutant enzyme by nickel affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Renatured (Comment) | Organism |
---|---|---|
7.5.2.6 | purified recombinant His6-tagged MsbA is reconstituted into proteoliposomes, that contain phosphocholine, using detergent destabilization | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
7.5.2.6 | additional information | - |
protein quantification by labeling of the purified native protein with MIANS, i.e. 2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid, on Cys315 | Salmonella enterica subsp. enterica serovar Typhimurium |
7.5.2.6 | 0.4 | - |
ATPase activity, purified recombinant enzyme, labeled or unlabeled with 2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.5.2.6 | ATP + H2O + Lipid A/in | MsbA contains two substrate-binding sites that communicate with both the nucleotide-binding domain and with each other, one is a high affinity binding site for the physiological substrate, lipid A, and the other site interacts with drugs with comparable affinity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + Lipid A/out | - |
? | |
7.5.2.6 | ATP + H2O + lipopolysaccharide/in | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + lipopolysaccharide/out | - |
? | |
7.5.2.6 | ATP + H2O + lipopolysaccharide/in | MsbA is an essential ABC transporter involved in lipid A transport across the cytoplasmic membrane | Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + lipopolysaccharide/out | - |
? | |
7.5.2.6 | additional information | binding of of amphipathic drugs, e.g. of daunorubicin, quercetin, verapamil, or propafenone GP12, alters the protein conformation | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.5.2.6 | ? | x * 65000, about, purified recombinant enzyme, mass spectrometry | Salmonella enterica subsp. enterica serovar Typhimurium |
7.5.2.6 | More | secondary structure analysis, binding of of amphipathic drugs, e.g. of daunorubicin, alters the protein conformation, topical enzyme model, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.5.2.6 | MsbA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.5.2.6 | additional information | - |
ligand binding assays at 23°C | Salmonella enterica subsp. enterica serovar Typhimurium |
7.5.2.6 | 37 | - |
ATPase assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.5.2.6 | 7.5 | - |
ATPase assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.5.2.6 | ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |