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Literature summary extracted from

  • Zamocky, M.; Furtmueller, P.G.; Bellei, M.; Battistuzzi, G.; Stadlmann, J.; Vlasits, J.; Obinger, C.
    Intracellular catalase/peroxidase from the phytopathogenic rice blast fungus Magnaporthe grisea: expression analysis and biochemical characterization of the recombinant protein (2009), Biochem. J., 418, 443-451.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli BL21 DE3 Star cells Pyricularia grisea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 4.8
-
 H2O2 at pH 6.0 and 25°C Pyricularia grisea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.21 extracellular
-
 Pyricularia grisea
-
-
1.11.1.21 intracellular
-
 Pyricularia grisea 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.21 83170
-
 theoretical molar mass of monomeric KatG1 with one heme b and a His6-tag Pyricularia grisea
1.11.1.21 88000
-
 4 * 88000, about 95% of the protein exists in homodimeric form, gel filtration Pyricularia grisea
1.11.1.21 90000
-
 2 * 90000, about 95% of the protein exists in homodimeric form, gel filtration Pyricularia grisea
1.11.1.21 160000
-
 dimeric KatG1, SDS-PAGE Pyricularia grisea
1.11.1.21 180000
-
 homodimer, gel filtration Pyricularia grisea
1.11.1.21 352000
-
 homotetramer, gel filtration Pyricularia grisea

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Pyricularia grisea A4QUT2 strain MA 829
-
1.11.1.21 Pyricularia grisea A4R5S9 strain MA 829
-
1.11.1.21 Pyricularia grisea ATCC MYA-4617 A4QUT2 strain MA 829
-
1.11.1.21 Pyricularia grisea ATCC MYA-4617 A4R5S9 strain MA 829
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 Ni-Sepharose column chromatography, Superdex 200 gel filtration Pyricularia grisea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pyricularia grisea ?
-
 ?
1.11.1.21 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pyricularia grisea ATCC MYA-4617 ?
-
 ?
1.11.1.21 ethanol + H2O2
-
 Pyricularia grisea acetaldehyde + ?
-
 ?
1.11.1.21 ethanol + H2O2
-
 Pyricularia grisea ATCC MYA-4617 acetaldehyde + ?
-
 ?
1.11.1.21 guaiacol + H2O2
-
 Pyricularia grisea ?
-
 ?
1.11.1.21 guaiacol + H2O2
-
 Pyricularia grisea ATCC MYA-4617 ?
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Pyricularia grisea ?
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Pyricularia grisea ATCC MYA-4617 ?
-
 ?
1.11.1.21 pyrogallol + H2O2
-
 Pyricularia grisea ?
-
 ?
1.11.1.21 pyrogallol + H2O2
-
 Pyricularia grisea ATCC MYA-4617 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.21 homodimer 2 * 90000, about 95% of the protein exists in homodimeric form, gel filtration Pyricularia grisea
1.11.1.21 homotetramer 4 * 88000, about 95% of the protein exists in homodimeric form, gel filtration Pyricularia grisea

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 catalase/peroxidase
-
 Pyricularia grisea
1.11.1.21 KatG
-
 Pyricularia grisea
1.11.1.21 KatG1 located intracellularly Pyricularia grisea
1.11.1.21 KatG2 located extracellularly Pyricularia grisea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.21 40 65 95% loss of catalase activity after 22 h at 40°C, 95% loss of catalase activity after 330 min at 45°C, 95% loss of catalase activity after 48 min at 50°C, 95% loss of catalase activity after 15 min at 55°C, 95% loss of catalase activity after 8 min at 60°C, 95% loss of catalase activity after 1 min at 65°C Pyricularia grisea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.21 7010
-
 H2O2 at pH 6.0 and 25°C Pyricularia grisea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 5 5.5 also acts as a versatile peroxidase with a pH optimum in the range 5.0-5.5, using both one-electron [2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), o-dianisidine, pyrogallol, or guaiacol] and two-electron (Br-, I- or ethanol) donors Pyricularia grisea
1.11.1.21 6
-
-
 Pyricularia grisea

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Pyricularia grisea
1.11.1.21 heme oxidoreductase with a predominant five-coordinated high-spin heme b, ferric MagKatG1 exhibits the typical bands of a heme b-containing peroxidase Pyricularia grisea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.21 Pyricularia grisea isoelectric focusing
-
 5.7
1.11.1.21 Pyricularia grisea calculated from amino acid sequence
-
 6