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Literature summary extracted from

  • Bebrone, C.; Anne, C.; Kerff, F.; Garau, G.; De Vriendt, K.; Lantin, R.; Devreese, B.; Van Beeumen, J.; Dideberg, O.; Frere, J.; Galleni, M.
    Mutational analysis of the zinc- and substrate-binding sites in the CphA metallo-beta -lactamase from Aeromonas hydrophila (2008), Biochem. J., 414, 151-159.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli Aeromonas hydrophila

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 D120A site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 D120T site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 H118A site-directed mutant of CphA, generated and characterized, H118A and H196A mutations lead to a decrease of at least 1000fold in activity against carbapenems compared with the wild-type enzyme Aeromonas hydrophila
3.5.2.6 H196A site-directed mutant of CphA, generated and characterized, H118A and H196A mutations lead to a decrease of at least 1000fold in activity against carbapenems compared with the wild-type enzyme Aeromonas hydrophila
3.5.2.6 H263A site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 K224Q site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 K226Q site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 N116H/N220G site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 N116H/N220G/K224Q site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 T157A site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 V67A site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 V67D site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 V67I site-directed mutant of CphA, generated and characterized Aeromonas hydrophila
3.5.2.6 V67LFKHV site-directed mutant of CphA, generated and characterized Aeromonas hydrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 EDTA inactivation of CphA wild-type and the H118A, H196A, H263A and D120A mutants by EDTA is studied in 15 mM sodium cacodylate (pH 6.5) at 30°C in the presence of different concentrations of EDTA Aeromonas hydrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.004
-
 nitrocefin N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.015
-
 nitrocefin H196A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.015
-
 nitrocefin H263A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.026
-
 nitrocefin D120A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.026
-
 nitrocefin V67A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.03
-
 nitrocefin H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.032
-
 nitrocefin N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.036
-
 nitrocefin V67D mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.05
-
 nitrocefin D120T mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.05
-
 cefotaxime N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.05
-
 nitrocefin V67I mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.053
-
 cephaloridine N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.1
-
 cefotaxime above, wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.1
-
 biapenem D120A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.1
-
 biapenem N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.1
-
 biapenem V67A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.11
-
 nitrocefin T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.135
-
 nitrocefin K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.14
-
 cefotaxime K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.145
-
 cephaloridine N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.15
-
 benzylpenicillin N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.17
-
 biapenem wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.18
-
 Imipenem V67I mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.185
-
 Imipenem N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.21
-
 Imipenem D120T mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.21
-
 benzylpenicillin H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.22
-
 Imipenem V67A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.3
-
 Imipenem N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.31
-
 Imipenem V67D mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.33
-
 Imipenem D120A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.34
-
 Imipenem wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.38
-
 Imipenem K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.39
-
 biapenem N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.434
-
 cefotaxime N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.45
-
 cefotaxime T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.55
-
 benzylpenicillin T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.57
-
 Imipenem T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.7
-
 cefotaxime K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.73
-
 biapenem H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.73
-
 benzylpenicillin V67I mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.8
-
 benzylpenicillin K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.84
-
 biapenem K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.85
-
 nitrocefin K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.87
-
 benzylpenicillin wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.92
-
 benzylpenicillin N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1
-
 cephaloridine K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1
-
 benzylpenicillin V67A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.15
-
 Imipenem H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.3
-
 nitrocefin wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.5
-
 biapenem above, H263A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.5
-
 Imipenem above, K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.5
-
 biapenem above, K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.5
-
 biapenem above, T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.5
-
 biapenem above, V67D mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.5
-
 biapenem above, V67I mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.5
-
 Imipenem above, V67LFKHV mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.5
-
 biapenem above, V67LFKHV mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.7
-
 Imipenem H263A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1.7
-
 cephaloridine K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 2
-
 nitrocefin above, V67LFKHV mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 4.3
-
 Imipenem H196A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 4.5
-
 cephaloridine above, H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 4.5
-
 cephaloridine above, H196A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 5
-
 benzylpenicillin above, H196A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 5
-
 benzylpenicillin above, K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 5
-
 benzylpenicillin above, V67D mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 6
-
 cephaloridine wild-type, 30°C, pH 6.5 Aeromonas hydrophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zn2+ zinc content is determined by ICP-MS. Position of the second inhibitory zinc ion has not been identified Aeromonas hydrophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 25120
-
 H118A mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25120
-
 H196A mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25120
-
 H263A mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25120
-
 H263A mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25130
-
 H118A mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25130
-
 H196A mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25150
-
 D120A mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25150
-
 D120A mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25160
-
 N116H/R121H/K224Q mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25160
-
 N116H/R121H/K224Q mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25160
-
 T157A mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25160
-
 T157A mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25160
-
 V67A mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25160
-
 V67A mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25180
-
 D120T mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25180
-
 D120T mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25190
-
 H118A mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25190
-
 H196A mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25190
-
 K224Q mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25190
-
 K224Q mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25190
-
 K226Q mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25190
-
 K226Q mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25190
-
 wild-type, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25190
-
 wild-type, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25200
-
 V67I mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25200
-
 V67I mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25210
-
 D120A mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25220
-
 H263A mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25220
-
 N116H/R121H/K224Q mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25220
-
 T157A mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25220
-
 V67A mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25240
-
 D120T mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25250
-
 K224Q mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25250
-
 K226Q mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25250
-
 wild-type, native, measured mass Aeromonas hydrophila
3.5.2.6 25270
-
 V67I mutant, native, measured mass Aeromonas hydrophila
3.5.2.6 25810
-
 V67LFKHV mutant, denatured, measured mass Aeromonas hydrophila
3.5.2.6 25880
-
 V67LFKHV mutant, native, measured mass, 1 Zn2+-ion Aeromonas hydrophila
3.5.2.6 25900
-
 V67LFKHV mutant, denatured, calculated mass Aeromonas hydrophila
3.5.2.6 25940
-
 V67LFKHV mutant, native, measured mass, 2 Zn2+-ions Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Aeromonas hydrophila P26918
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Aeromonas hydrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 benzylpenicillin + H2O
-
 Aeromonas hydrophila (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 biapenem + H2O
-
 Aeromonas hydrophila (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate
-
 ?
3.5.2.6 carbapenem + H2O
-
 Aeromonas hydrophila [(2R)-2,3-dihydro-1H-pyrrol-2-yl]acetic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Aeromonas hydrophila (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Aeromonas hydrophila (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 imipenem + H2O
-
 Aeromonas hydrophila (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Aeromonas hydrophila (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 More His118 and His196 participate in the catalytic mechanism of the subclass B2 CphA metallo-beta-lactamase, for which an intact Asp120/Cys221/His263 site is essential Aeromonas hydrophila

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 carbapenemase
-
 Aeromonas hydrophila
3.5.2.6 CphA
-
 Aeromonas hydrophila
3.5.2.6 metallo-beta-lactamase
-
 Aeromonas hydrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.0002
-
 cefotaxime above, wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.0002
-
 nitrocefin D120T mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.0006
-
 cefotaxime K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.001
-
 cefotaxime K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.0015
-
 biapenem D120A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.0024
-
 nitrocefin D120A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.003
-
 nitrocefin H263A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.004
-
 benzylpenicillin K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.004
-
 benzylpenicillin V67A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.004
-
 nitrocefin V67D mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.004
-
 nitrocefin V67I mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.005
-
 biapenem above, H263A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.005
-
 nitrocefin H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.005
-
 nitrocefin V67A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.006
-
 nitrocefin H196A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.006
-
 nitrocefin above, V67LFKHV mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.006
-
 cephaloridine below, wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.006
-
 cephaloridine K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.007
-
 benzylpenicillin H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.007
-
 nitrocefin K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.008
-
 nitrocefin wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.01
-
 cephaloridine above, H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.01
-
 nitrocefin K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.015
-
 cefotaxime N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.015
-
 nitrocefin T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.02
-
 Imipenem H263A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.023
-
 cefotaxime T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.024
-
 Imipenem D120A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.024
-
 cephaloridine K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.025
-
 Imipenem D120T mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.03
-
 benzylpenicillin wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.03
-
 benzylpenicillin above, K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.03
-
 benzylpenicillin above, V67D mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.03
-
 cephaloridine N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.08
-
 benzylpenicillin V67I mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.1
-
 benzylpenicillin above, H196A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.1
-
 biapenem H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.11
-
 cephaloridine above, H196A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.13
-
 benzylpenicillin N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.2
-
 nitrocefin N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.26
-
 biapenem N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.3
-
 cefotaxime N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.7
-
 nitrocefin N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.9
-
 cephaloridine N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 0.9
-
 Imipenem N116H/N220G/K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1
-
 benzylpenicillin T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 2.4
-
 biapenem above, V67LFKHV mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 2.4
-
 Imipenem H118A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 3.3
-
 benzylpenicillin N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 5
-
 biapenem N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 16
-
 Imipenem H196A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 16
-
 Imipenem N116H/N220G mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 30
-
 Imipenem above, V67LFKHV mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 50
-
 biapenem above, V67I mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 60
-
 biapenem above, K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 100
-
 biapenem above, V67D mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 100
-
 biapenem V67A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 130
-
 biapenem above, T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 300
-
 biapenem wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 490
-
 Imipenem above, K224Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1000
-
 Imipenem K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1000
-
 biapenem K226Q mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1000
-
 Imipenem V67A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1200
-
 Imipenem wild-type, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1280
-
 Imipenem V67D mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1680
-
 Imipenem T157A mutant, 30°C, pH 6.5 Aeromonas hydrophila
3.5.2.6 1770
-
 Imipenem V67I mutant, 30°C, pH 6.5 Aeromonas hydrophila