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Literature summary extracted from

  • Osoata, G.O.; Yamamura, S.; Ito, M.; Vuppusetty, C.; Adcock, I.M.; Barnes, P.J.; Ito, K.
    Nitration of distinct tyrosine residues causes inactivation of histone deacetylase 2 (2009), Biochem. Biophys. Res. Commun., 384, 366-371.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.98 medicine HDAC2 is nitrated under nitrative/oxidative stress and in the peripheral lung tissues of smokers and patients with chronic obstructive pulmonary disease Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.98 Y146A mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation Homo sapiens
3.5.1.98 Y146A in presence of peroxynitrite, activity is reduced by 32%. In presence of 3-morpholinosydonomine, activity is reduced similar to wild-type Homo sapiens
3.5.1.98 Y153A mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation Homo sapiens
3.5.1.98 Y153A in presence of 3-morpholinosydonomine, activity is reduced similar to wild-type. In presence of peroxynitrite, activity is abolished Homo sapiens
3.5.1.98 Y253A mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation Homo sapiens
3.5.1.98 Y253A Y253 is at least partly responsible for the proteasomal degradation of HDAC2 under nitrative stress. Activity is not reduced in presence of 3-morpholinosydonomine Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.98 additional information nitration of distinct tyrosine residues; nitrative/oxidative stress reduce HDAC2 expression via nitration of distinct tyrosine residues. Peroxynitrite, hydrogen peroxide and cigarette smoke-conditioned medium reduce HDAC2 expression in A549 epithelial cells in vitro. This reduction is due to increased proteasomal degradation following ubiquitination rather than reduction of mRNA expression or stability Homo sapiens
3.5.1.98 peroxynitrite activity of wild-type enzyme is reduced to 38% in presence of peroxynitrite. Activities of mutants Y153A and Y253A are 233 completely abolished in the presence of peroxynitrite. Mutant Y146A shows 32% reduction in activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.98 nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.98 65000
-
 determined by SDS-PAGE and Western Blot analysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.98 Homo sapiens
-
-
-
3.5.1.98 Homo sapiens Q92769
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.98 A-549 cell
-
 Homo sapiens
-
3.5.1.98 lung
-
 Homo sapiens
-
3.5.1.98 U2-OS cell
-
 Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
3.5.1.98 HDAC2
-
 Homo sapiens
3.5.1.98 histone deacetylase 2
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.1.98 metabolism nitrative/oxidative stress reduce HDAC2 expression via nitration of distinct tyrosine residues. Peroxynitrite, hydrogen peroxide and cigarette smoke-conditioned medium reduce HDAC2 expression in A549 epithelial cells in vitro. This reduction is due to increased proteasomal degradation following ubiquitination rather than reduction of mRNA expression or stability Homo sapiens