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Literature summary extracted from

  • Goda, H.M.; Ushigusa, K.; Ito, H.; Okino, N.; Narimatsu, H.; Ito, M.
    Molecular cloning, expression, and characterization of a novel endo-alpha-N-acetylgalactosaminidase from Enterococcus faecalis (2008), Biochem. Biophys. Res. Commun., 375, 541-546.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.97 into vector pET23a and expressed with a histidine-tag in Escherichia coli BL21(DE3) Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.97 Cu2+ 1 mM greatly inhibits Enterococcus faecalis
3.2.1.97 additional information 1 mM of Mg2+, Ni2+, Ca2+, Co2+, Na+, Mn2+, Fe2+, Fe3+, EGTA, and EDTA have little effect on the enzyme activity Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.97 0.0596
-
 Galbeta1-3GalNAcalpha-pNP
-
 Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.97 150000
-
 1 * 150000, SDS-PAGE Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.97 Enterococcus faecalis Q833X5
-
-
3.2.1.97 Enterococcus faecalis NBRC3971 Q833X5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.97 by gel filtration Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.97 4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
-
 Enterococcus faecalis 4-nitrophenol + N-acetyl-alpha-D-galactosamine
-
 ?
3.2.1.97 4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
-
 Enterococcus faecalis NBRC3971 4-nitrophenol + N-acetyl-alpha-D-galactosamine
-
 ?
3.2.1.97 Galbeta1-3GalNAcalpha-pNP + H2O
-
 Enterococcus faecalis Galbeta1-3GalNAc + p-nitrophenol
-
 ?
3.2.1.97 Galbeta1-3GalNAcalpha-pNP + H2O
-
 Enterococcus faecalis NBRC3971 Galbeta1-3GalNAc + p-nitrophenol
-
 ?
3.2.1.97 Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O
-
 Enterococcus faecalis ?
-
 ?
3.2.1.97 Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O
-
 Enterococcus faecalis NBRC3971 ?
-
 ?
3.2.1.97 Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O
-
 Enterococcus faecalis ?
-
 ?
3.2.1.97 Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O
-
 Enterococcus faecalis NBRC3971 ?
-
 ?
3.2.1.97 Glc-NAcbeta1-3GalNAcalpha1-pNP + H2O
-
 Enterococcus faecalis ?
-
 ?
3.2.1.97 additional information can not release the oligosaccharide from GlcNAcbeta1-6GalNAcalpha1-pNP Enterococcus faecalis ?
-
 ?
3.2.1.97 additional information can not release the oligosaccharide from GlcNAcbeta1-6GalNAcalpha1-pNP Enterococcus faecalis NBRC3971 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.97 monomer 1 * 150000, SDS-PAGE Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.97 endo-alpha-N-acetylgalactosaminidase
-
 Enterococcus faecalis
3.2.1.97 endo-EF
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.97 45
-
-
 Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.97 49.9
-
 Galbeta1-3GalNAcalpha-pNP
-
 Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.97 6
-
-
 Enterococcus faecalis