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Literature summary extracted from

  • Tang, H.K.; Tang, H.Y.; Hsu, S.C.; Chu, Y.R.; Chien, C.H.; Shu, C.H.; Chen, X.
    Biochemical properties and expression profile of human prolyl dipeptidase DPP9 (2009), Arch. Biochem. Biophys., 485, 120-127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.5 DPP9 is expressed in baculovirus-infected Sf9 insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.14.5 F822A the DPP8 mutation abolishes the enzymatic activity without disrupting its quaternary structure Homo sapiens
3.4.14.5 F842A the DPP9 mutation abolishes the enzymatic activity without disrupting its quaternary structure Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 additional information when assayed against L-Ala-L-Pro-p-nitroanilide or Gly-L-Pro-p-nitroanilide, the activity of DPP9 is not affected by the presence of reducing agents, such as 1 mM dithiothreitol or 18 mM beta-mercaptoethanol Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.5 0.008
-
 L-Lys-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.014
-
 L-Leu-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.014
-
 L-Arg-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.018
-
 L-Met-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.073
-
 L-Lys-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.099
-
 L-Ala-L-Pro-p-nitroanilide wild type , in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.102
-
 Gly-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.127
-
 L-Leu-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.189
-
 L-Ser-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.337
-
 L-Asn-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.432
-
 L-Ala-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
3.4.14.5 1.244
-
 L-Glu-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.5 cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 95000
-
 2 * 95000, SDS-PAGE Homo sapiens
3.4.14.5 189000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5 Strep-Tactin resin purification system Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.5 blood
-
 Homo sapiens
-
3.4.14.5 epithelial cell
-
 Homo sapiens
-
3.4.14.5 fibroblast
-
 Homo sapiens
-
3.4.14.5 PBMC cell line
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 0.01
-
 crude extract, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.8
-
 after 80fold purification, in PBS, pH 8.0 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.4.14.5 -80°C, purified enzyme, 1 month, no loss of activity Homo sapiens
3.4.14.5 22°C, purified enzyme, 12 h, no loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Gly-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Lys-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Ala-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Ala-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Arg-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Arg-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Asn-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Asn-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Asp-L-Pro-p-nitroanilide + H2O least preferred substrate Homo sapiens L-Asp-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Glu-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Glu-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Leu-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Leu-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Lys-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Lys-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Lys-L-Pro-p-nitroanilide + H2O most preferred substrate Homo sapiens L-Lys-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Met-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Met-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 L-Ser-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Ser-L-Pro + p-nitroaniline
-
 ?
3.4.14.5 additional information no endopeptidase or tripeptidase activity is detected for DPP9, tested against Z-Gly-L-Pro-p-nitroanilide, L-Ala-L-Ala-L-Ala-p-nitroanilide, and L-Ala-L-Ala-L-Pro-p-nitroanilide Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 homodimer 2 * 95000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.14.5 dipeptidyl peptidase 8
-
 Homo sapiens
3.4.14.5 dipeptidyl peptidase 9
-
 Homo sapiens
3.4.14.5 DPP8 DPP8 is a prolyl dipeptidases, cleaving the peptide bond after the penultimate proline residue Homo sapiens
3.4.14.5 DPP9 DPP9 is a prolyl dipeptidases, cleaving the peptide bond after the penultimate proline residue Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.5 7.5 8 the activity decreases above pH 8.0 or below pH 7.5 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.5 0.4
-
 L-Lys-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
3.4.14.5 0.6
-
 L-Ala-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
3.4.14.5 1.9
-
 L-Leu-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
3.4.14.5 5
-
 L-Lys-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 6.3
-
 L-Leu-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 7
-
 L-Glu-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 7.7
-
 L-Arg-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 8.7
-
 L-Met-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 11
-
 L-Asn-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 18
-
 L-Ser-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 20.3
-
 Gly-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
3.4.14.5 33
-
 L-Ala-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7.5 8
-
 Homo sapiens