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Literature summary extracted from

  • Liu, G.; Zhou, J.; Jin, R.; Zhou, M.; Wang, J.; Lu, H.; Qu, Y.
    Enhancing survival of Escherichia coli by expression of azoreductase AZR possessing quinone reductase activity (2008), Appl. Microbiol. Biotechnol., 80, 409-416.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6 into the vector pGEX 4T-1 for transformation into Escherichia coli JM109 cells Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.6 dicoumarol
-
 Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.0133
-
 anthraquinone-2-sulfonate
-
 Cereibacter sphaeroides
1.7.1.6 0.0161
-
 anthraquinone-2,6-disulfonate
-
 Cereibacter sphaeroides
1.7.1.6 0.0162
-
 2-methyl-1,4-naphthoquinone
-
 Cereibacter sphaeroides
1.7.1.6 0.0346
-
 2-Hydroxy-1,4-naphthoquinone
-
 Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Cereibacter sphaeroides Q8GKS3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 2-hydroxy-1,4-naphthoquinone + NADPH + H+ lawsone, quinone reductase activity Cereibacter sphaeroides ?
-
 ?
1.7.1.6 2-methyl-1,4-naphthoquinone + NADPH + H+ menadione, quinone reductase activity Cereibacter sphaeroides ?
-
 ?
1.7.1.6 anthraquinone-2,6-disulfonate + NADPH + H+ quinone reductase activity Cereibacter sphaeroides ?
-
 ?
1.7.1.6 anthraquinone-2-sulfonate + NADPH + H+ quinone reductase activity Cereibacter sphaeroides ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 azoreductase
-
 Cereibacter sphaeroides
1.7.1.6 AZR
-
 Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 50
-
 substrate 2-methyl-1,4-naphthoquinone, quinone reductase activity Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.6 211.2
-
 anthraquinone-2,6-disulfonate
-
 Cereibacter sphaeroides
1.7.1.6 218.4
-
 anthraquinone-2-sulfonate
-
 Cereibacter sphaeroides
1.7.1.6 345
-
 2-Hydroxy-1,4-naphthoquinone
-
 Cereibacter sphaeroides
1.7.1.6 3278
-
 2-methyl-1,4-naphthoquinone
-
 Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 7
-
 quinone reductase activity Cereibacter sphaeroides
1.7.1.6 8.5
-
 substrate 2-methyl-1,4-naphthoquinone, quinone reductase activity Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.6 8 9 maximal activity Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 NADPH
-
 Cereibacter sphaeroides

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.6 0.0876
-
 dicoumarol
-
 Cereibacter sphaeroides