Any feedback?
Literature summary extracted from
- Characterization of a modular enzyme of exo-1,5-alpha-L-arabinofuranosidase and arabinan binding module from Streptomyces avermitilis NBRC14893 (2008), Appl. Microbiol. Biotechnol., 80, 399-408.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.55 | expression in Escherichia coli BL21 (DE3) | Streptomyces avermitilis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 35000 | - |
SDS-PAGE, CBM42-deleted mutant, predicted molecular mass 33000 Da | Streptomyces avermitilis |
| 3.2.1.55 | 53000 | - |
SDS-PAGE, recombinant SaAraf43A, predicted molecular mass 52000 Da | Streptomyces avermitilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.55 | Streptomyces avermitilis | Q82P90 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.55 | Ni-nitrilotriacetic acid (NTA) agarose column chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) | Streptomyces avermitilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 2.92 | - |
p-nitrophenyl alpha-L-arabinofuranoside, alpha-L-arabinofuranoside | Streptomyces avermitilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.55 | 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces avermitilis | alpha-L-arabinofuranose | - |
? |  |
| 3.2.1.55 | debrached 1,5-arabinan + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
| 3.2.1.55 | additional information | does not hydrolyse arabinoxylan, arabinogalactan, gum arabic, and arabinan | Streptomyces avermitilis | ? | - |
? | |
| 3.2.1.55 | additional information | does not hydrolyse methyl 2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside, methyl 3-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | Streptomyces avermitilis | ? | - |
? | |
| 3.2.1.55 | additional information | does not hydrolyze p-nitrophenyl-alpha-L-arabinopyranoside, p-nitrophenyl-beta-D-xylopyranoside, or p-nitrophenyl-beta-D-galactopyranoside | Streptomyces avermitilis | ? | - |
? | |
| 3.2.1.55 | p-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces avermitilis | p-nitrophenol + L-arabinofuranose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.55 | alpha-L-arabinofuranosidase | - |
Streptomyces avermitilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 45 | - |
- |
Streptomyces avermitilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 30 | - |
more than 80% activity is retained after incubation at 30°C for 60 min | Streptomyces avermitilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 6 | - |
- |
Streptomyces avermitilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 5 | 6.5 | - |
Streptomyces avermitilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 5 | 6.5 | 30°C, more than 80% of the activity is retained | Streptomyces avermitilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
